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- W2405314364 abstract "An attenuated Derzsy's disease virus strain, designated BAV, was studied in goose embryos. A total of 248 embryonated goose eggs, coming from a susceptible laying flock with no yolk-derived immunity (group I) and from a vaccinated laying flock (group II) were used. The eggs were inoculated into the allantoic cavity with 10(1.9), 10(2.9) or 10(3.9) EID 50/0.2 ml virus on day 12 or day 20 of incubation. Embryos were killed at 5-day intervals. The dead embryos and the hatched goslings (up to 2 weeks of age) were examined by gross and histopathological methods. Reisolation of the virus from the organs was attempted, serum samples were tested for the presence of antibodies, and lymphocytes separated from the circulating blood were used in the lymphocyte stimulation and immunorosette formation tests. Embryos of both groups I and II, inoculated at either time of incubation, showed a body mass gain inferior to that of the controls. Sixteen (group I) and 12 (group II) of the embryos inoculated on day 12 of incubation died. Some (group I: 15, group II: 6) of the embryos inoculated on day 20 of incubation failed to hatch. The pathomorphological changes seen in the embryos killed between days 17 and 22 of incubation were of degenerative character. In embryos killed later (between days 23 and 58 of incubation) the degenerative changes were accompanied by infiltration by inflammatory cells. Reisolation of the virus strain was mostly successful between postinoculation (PI) days 5 and 10. Specific virus-neutralizing antibodies and cellular immune response were demonstrable already at hatching.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
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- W2405314364 date "1991-01-01" @default.
- W2405314364 modified "2023-10-16" @default.
- W2405314364 title "Pathological and immunological study of goose embryos and goslings inoculated with an attenuated strain of Derzsy's disease virus." @default.
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