Matches in SemOpenAlex for { <https://semopenalex.org/work/W2405641014> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2405641014 endingPage "308" @default.
- W2405641014 startingPage "305" @default.
- W2405641014 abstract "Objective To test the in vitro susceptibility to macrolides of urogenital Chlamydia trachomatis (Ct) isolates, screen resistant Ct strains, and to explore resistance mechanism at the molecular level. Methods A total of 42 Ct strains were isolated from cervical or urethral swab samples and propagated in McCoy cells until the infection rate reached more than 90%. Then, susceptibility test was performed to evaluate the activity of three macrolides. Reverse transcription PCR and PCR were used to amplify two macrolide-resistance related genes, i.e., 23S rRNA gene and L4 gene, respectively in 2 erythromycin-resistant Ct strains and 4 erythromycin-sensitive strains followed by direct sequencing. Results The minimal inhibitory concentration (MIC) varied from 0.5 to 2 mg/L for erythromycin, 0.008 to 0.032 mg/L for clarithromycin, 0.125 to 0.5 mg/Lfor azithromycin. Erythromycin resistance was found in 2 isolates with the MIC value being 2 mg/L. Two mutations, C2452A and T2611A/C (Escherichia coli numbering) in the 23S rRNA gene, were detected in the resistant strains only, while the other 2 mutations, Pro113Leu and Pro156 Ala in L4 gene, were observed in all the tested strains. Conclusions Erythromycin-resistant Ct strains have emerged in clinical settings. The low-level erythromycin resistance may be associated with C2452A and T261 1C mutations in the 23S rRNA gene, whereas the point mutations in L4 gene is unlikely related to erythromycin resistance.Key words: Chlamydia trachomatis; Macrolides; Microbial sensitivity tests; Resistance gene" @default.
- W2405641014 created "2016-06-24" @default.
- W2405641014 creator A5007735191 @default.
- W2405641014 creator A5012387801 @default.
- W2405641014 creator A5023543349 @default.
- W2405641014 creator A5025116090 @default.
- W2405641014 creator A5042100539 @default.
- W2405641014 creator A5069448029 @default.
- W2405641014 date "2010-05-15" @default.
- W2405641014 modified "2023-09-23" @default.
- W2405641014 title "In vitro sensitivity to macrolides and mutation analysis in the 23S rRNA gene of Chlamydia trachomatis." @default.
- W2405641014 doi "https://doi.org/10.3760/cma.j.issn.0412-4030.2010.05.004" @default.
- W2405641014 hasPublicationYear "2010" @default.
- W2405641014 type Work @default.
- W2405641014 sameAs 2405641014 @default.
- W2405641014 citedByCount "0" @default.
- W2405641014 crossrefType "journal-article" @default.
- W2405641014 hasAuthorship W2405641014A5007735191 @default.
- W2405641014 hasAuthorship W2405641014A5012387801 @default.
- W2405641014 hasAuthorship W2405641014A5023543349 @default.
- W2405641014 hasAuthorship W2405641014A5025116090 @default.
- W2405641014 hasAuthorship W2405641014A5042100539 @default.
- W2405641014 hasAuthorship W2405641014A5069448029 @default.
- W2405641014 hasConcept C104317684 @default.
- W2405641014 hasConcept C153911025 @default.
- W2405641014 hasConcept C159047783 @default.
- W2405641014 hasConcept C176947019 @default.
- W2405641014 hasConcept C2777391075 @default.
- W2405641014 hasConcept C2778907293 @default.
- W2405641014 hasConcept C2780206985 @default.
- W2405641014 hasConcept C40749294 @default.
- W2405641014 hasConcept C501593827 @default.
- W2405641014 hasConcept C54355233 @default.
- W2405641014 hasConcept C67705224 @default.
- W2405641014 hasConcept C86803240 @default.
- W2405641014 hasConcept C88478588 @default.
- W2405641014 hasConcept C89423630 @default.
- W2405641014 hasConceptScore W2405641014C104317684 @default.
- W2405641014 hasConceptScore W2405641014C153911025 @default.
- W2405641014 hasConceptScore W2405641014C159047783 @default.
- W2405641014 hasConceptScore W2405641014C176947019 @default.
- W2405641014 hasConceptScore W2405641014C2777391075 @default.
- W2405641014 hasConceptScore W2405641014C2778907293 @default.
- W2405641014 hasConceptScore W2405641014C2780206985 @default.
- W2405641014 hasConceptScore W2405641014C40749294 @default.
- W2405641014 hasConceptScore W2405641014C501593827 @default.
- W2405641014 hasConceptScore W2405641014C54355233 @default.
- W2405641014 hasConceptScore W2405641014C67705224 @default.
- W2405641014 hasConceptScore W2405641014C86803240 @default.
- W2405641014 hasConceptScore W2405641014C88478588 @default.
- W2405641014 hasConceptScore W2405641014C89423630 @default.
- W2405641014 hasIssue "5" @default.
- W2405641014 hasLocation W24056410141 @default.
- W2405641014 hasOpenAccess W2405641014 @default.
- W2405641014 hasPrimaryLocation W24056410141 @default.
- W2405641014 hasRelatedWork W190645180 @default.
- W2405641014 hasRelatedWork W1981265857 @default.
- W2405641014 hasRelatedWork W1988626387 @default.
- W2405641014 hasRelatedWork W2010809035 @default.
- W2405641014 hasRelatedWork W2041846071 @default.
- W2405641014 hasRelatedWork W2105831490 @default.
- W2405641014 hasRelatedWork W2109875381 @default.
- W2405641014 hasRelatedWork W2118076162 @default.
- W2405641014 hasRelatedWork W2119617984 @default.
- W2405641014 hasRelatedWork W2128342254 @default.
- W2405641014 hasRelatedWork W2135191183 @default.
- W2405641014 hasRelatedWork W2166670043 @default.
- W2405641014 hasRelatedWork W2195444904 @default.
- W2405641014 hasRelatedWork W2357730508 @default.
- W2405641014 hasRelatedWork W2368359323 @default.
- W2405641014 hasRelatedWork W2378314613 @default.
- W2405641014 hasRelatedWork W2379417187 @default.
- W2405641014 hasRelatedWork W2387240254 @default.
- W2405641014 hasRelatedWork W2463427253 @default.
- W2405641014 hasRelatedWork W2586748395 @default.
- W2405641014 hasVolume "43" @default.
- W2405641014 isParatext "false" @default.
- W2405641014 isRetracted "false" @default.
- W2405641014 magId "2405641014" @default.
- W2405641014 workType "article" @default.