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- W240590310 abstract "Heliconias are species with big goals in cut flowers market. It arousesinterest in research about vigorous, fast and big scale of vegetativepropagation, in that way micropropagation could be an option. Moreoverplants propagated maintain several characteristic of interest tosatisfy the exigencies of producers. Cross section system or thin cellslayer “TCLs” is an important tool for in vitro plantculture, due to few vegetative materials that is required for theprocess and it is possible to obtain big quantity of germoplasm. Inthis way, were established the appropriated conditions for in vitropropagation of in Heliconia psittacorum choconiana variety, throughthis system; Thin cross sections of 1 mm of thickness was obtained ofpseudostems in this specie and cultivated with optimal response inMurashige and Skoog culture medium (MS, 1962), supplemented withtiamine 1 mg L-1, piridoxine 1 mg L-1, Nicotinic acid 1 mg L-1,Myoinositol 100 mg L-1, activated charcoal 0.5 g L-1, 2,4-D 1 mg L-1,BAP 1 mg L-1 hidrolizated Casein 1 g L-1 and Gelrite 1 g L-1 likegelificant agent, the conditions of culture were at 25 ± 1° Cof temperature and 16h light daily. The final result was the inductionsof direct organogenesis that permit obtain vigorous microplants ofheliconia, after eleven weeks. In conclusion, the technique of thincross section, is a good tool to grow sections of rhizome." @default.
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- W240590310 date "2009-01-01" @default.
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- W240590310 title "In vitro regeneration of Heliconia psittacorum, choconiana variety using thin cell layer (tcls) culture system." @default.
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