FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2406058765> ?p ?o ?g. }

• W2406058765 endingPage "6" @default.
• W2406058765 startingPage "1" @default.
• W2406058765 abstract "In the previous paper, Ishikawa reported that putrescine is always observed in the blood of lepromatous leprosy patient with M. leprae in his skin (LA LEPRO 36, 238 (1967)). It is well known that putrescine is one of the ptomains and causes the ptomaine poisoning. So the Ishikawa's report presents a serious problem confronting the lepromatous leorosy patients. In this paper we described the method of determination of putrescine and cadaverine in serum and then the result of measuring these ptomaines in the serum from lepromatous leprosy patients.The determination of putrescine and cadaverine is as follows; Serum was extracted with n-butanol by McIntire's method. Butanol layer was acidified with 1N-HCl and then butanol was removed by lyophilization. Lyophilized residue was applied to a small column of Amberlite CG-50 according to the method of D. R. Morris with some modification (cf. Fig. 1).By the use of Amberlite CG-50 putrescine and cadaverine could not mutually be separated, but could be separated from lysine, histidine, arginine, istamine etc. Standard curve of ninhydrin reaction showed that the concentration of putrescine or cadaverine was in proportion to the rate of ninhydrin reaction. The recovery of putrescine in normal human serum after butanol extraction and column chromatography was about 90%.About 2ml (varying from 1.6 to 4.5ml) of serum from lepromatous leprosy patient with bacilli in his skin was tested for detection of putrescine and/or cadaverine, but in no case out of seven we did find more than 0.05μ moles of putrescine and/or cadaverine in the serum.Ishikawa obtained the serum from 5ml of blood, so about 2 to 2.5ml of serum was used for detection of putrescine. The quantity of serum used for determination of putrescine by Ishikawa is almost the same as used by us. We do not believe that the Ishikawa's method is far better than that used by us.Putrescine and cadaverine are known to be formed by decarboxylation from ornithine and lysine respectively in living organisms. From our result we can not decide whether M. leprae has decarboxylase activity or not, because the products of this bacillus may be metabolized to non-toxic materials by human nzyme system to maintaine the homeostasis. For example, even if the putrescine is produced, it will perhaps be oxidized by diamine oxidase in human liver. It seems to be necessary to measure directly decarboxylase activity in the bacillus." @default.
• W2406058765 created "2016-06-24" @default.
• W2406058765 creator A5051813285 @default.
• W2406058765 creator A5076829080 @default.
• W2406058765 date "1972-01-01" @default.
• W2406058765 modified "2023-09-30" @default.
• W2406058765 title "Determination of Putrescine and Cadaverine in the Serum from Lepromatous Leprosy Patients" @default.
• W2406058765 cites W160140053 @default.
• W2406058765 cites W1995185952 @default.
• W2406058765 cites W2005555811 @default.
• W2406058765 cites W2058613232 @default.
• W2406058765 cites W65066218 @default.
• W2406058765 doi "https://doi.org/10.5025/hansen1930.41.1_1" @default.
• W2406058765 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4681799" @default.
• W2406058765 hasPublicationYear "1972" @default.
• W2406058765 type Work @default.
• W2406058765 sameAs 2406058765 @default.
• W2406058765 citedByCount "0" @default.
• W2406058765 crossrefType "journal-article" @default.
• W2406058765 hasAuthorship W2406058765A5051813285 @default.
• W2406058765 hasAuthorship W2406058765A5076829080 @default.
• W2406058765 hasBestOaLocation W24060587651 @default.
• W2406058765 hasConcept C150394285 @default.
• W2406058765 hasConcept C178790620 @default.
• W2406058765 hasConcept C181199279 @default.
• W2406058765 hasConcept C185592680 @default.
• W2406058765 hasConcept C2776928777 @default.
• W2406058765 hasConcept C2777167505 @default.
• W2406058765 hasConcept C2777713963 @default.
• W2406058765 hasConcept C2778699723 @default.
• W2406058765 hasConcept C2778944976 @default.
• W2406058765 hasConcept C2780161600 @default.
• W2406058765 hasConcept C43617362 @default.
• W2406058765 hasConcept C515207424 @default.
• W2406058765 hasConcept C55493867 @default.
• W2406058765 hasConceptScore W2406058765C150394285 @default.
• W2406058765 hasConceptScore W2406058765C178790620 @default.
• W2406058765 hasConceptScore W2406058765C181199279 @default.
• W2406058765 hasConceptScore W2406058765C185592680 @default.
• W2406058765 hasConceptScore W2406058765C2776928777 @default.
• W2406058765 hasConceptScore W2406058765C2777167505 @default.
• W2406058765 hasConceptScore W2406058765C2777713963 @default.
• W2406058765 hasConceptScore W2406058765C2778699723 @default.
• W2406058765 hasConceptScore W2406058765C2778944976 @default.
• W2406058765 hasConceptScore W2406058765C2780161600 @default.
• W2406058765 hasConceptScore W2406058765C43617362 @default.
• W2406058765 hasConceptScore W2406058765C515207424 @default.
• W2406058765 hasConceptScore W2406058765C55493867 @default.
• W2406058765 hasIssue "1" @default.
• W2406058765 hasLocation W24060587651 @default.
• W2406058765 hasLocation W24060587652 @default.
• W2406058765 hasOpenAccess W2406058765 @default.
• W2406058765 hasPrimaryLocation W24060587651 @default.
• W2406058765 hasRelatedWork W1544790606 @default.
• W2406058765 hasRelatedWork W2008714359 @default.
• W2406058765 hasRelatedWork W2014089074 @default.
• W2406058765 hasRelatedWork W2018393717 @default.
• W2406058765 hasRelatedWork W2067537539 @default.
• W2406058765 hasRelatedWork W2365646474 @default.
• W2406058765 hasRelatedWork W2409624353 @default.
• W2406058765 hasRelatedWork W2994207739 @default.
• W2406058765 hasRelatedWork W3137655649 @default.
• W2406058765 hasRelatedWork W2143195485 @default.
• W2406058765 hasVolume "41" @default.
• W2406058765 isParatext "false" @default.
• W2406058765 isRetracted "false" @default.
• W2406058765 magId "2406058765" @default.
• W2406058765 workType "article" @default.