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• W2406221278 endingPage "589" @default.
• W2406221278 startingPage "569" @default.
• W2406221278 abstract "Modification of the replicative sliding clamp, PCNA, by monoubiquitin, polyubiquitin, and SUMO contributes to the processing of DNA damage during replication. In order to investigate the properties of the relevant conjugation enzymes, their interactions, substrate recognition, and the regulation of their activities, reconstitution of the modification reactions from purified components in vitro is an instructive exercise. Here we describe the purification of the relevant enzymes and accessory proteins from E. coli or S. cerevisiae as well as protocols for setting up small-scale ubiquitylation and sumoylation reactions with budding yeast PCNA. In addition, we provide a method for the purification of monoubiquitylated PCNA for further biochemical studies." @default.
• W2406221278 created "2016-06-24" @default.
• W2406221278 creator A5022968869 @default.
• W2406221278 creator A5077688118 @default.
• W2406221278 date "2012-01-01" @default.
• W2406221278 modified "2023-10-18" @default.
• W2406221278 title "In Vitro PCNA Modification Assays" @default.
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• W2406221278 doi "https://doi.org/10.1007/978-1-61779-998-3_37" @default.
• W2406221278 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22941628" @default.
• W2406221278 hasPublicationYear "2012" @default.
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