Matches in SemOpenAlex for { <https://semopenalex.org/work/W2406816195> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2406816195 endingPage "218" @default.
- W2406816195 startingPage "211" @default.
- W2406816195 abstract "AbstractThe Western blotting/sequencing technique using polyvinylidene difluoride (PVDF) membrane is one of the most popular technique for Edman sequencing. A protein sample is transferred from a 2D polyacrylamide gel electrophoresis (2D-PAGE) gel onto a PVDF membrane by electroblotting. The membrane carrying the protein is directly subjected to protein sequencing. If sequencing fails after a few cycles, the PVDF membrane is removed from the sequencer and treated with deblocking solution. If this attempt at sequencing fails, alternative methods such as the Cleveland method are required. Because the resolution of 2D-PAGE is high, the combined use of 2D-PAGE, Western blotting, and Edman sequencing often allow effective sequence determination of proteins that could not be easily purified by conventional column chromatography.Key WordsEdman sequencing2D-PAGEblocked proteindeblockingCleveland method" @default.
- W2406816195 created "2016-06-24" @default.
- W2406816195 creator A5050673369 @default.
- W2406816195 date "2006-11-08" @default.
- W2406816195 modified "2023-09-27" @default.
- W2406816195 title "Edman Sequencing of Proteins from 2D Gels" @default.
- W2406816195 cites W1606423922 @default.
- W2406816195 cites W1966945401 @default.
- W2406816195 cites W2028311028 @default.
- W2406816195 cites W2100837269 @default.
- W2406816195 cites W2164358316 @default.
- W2406816195 doi "https://doi.org/10.1385/1-59745-227-0:211" @default.
- W2406816195 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17093313" @default.
- W2406816195 hasPublicationYear "2006" @default.
- W2406816195 type Work @default.
- W2406816195 sameAs 2406816195 @default.
- W2406816195 citedByCount "3" @default.
- W2406816195 countsByYear W24068161952015 @default.
- W2406816195 countsByYear W24068161952020 @default.
- W2406816195 crossrefType "book-chapter" @default.
- W2406816195 hasAuthorship W2406816195A5050673369 @default.
- W2406816195 hasConcept C10010492 @default.
- W2406816195 hasConcept C101660505 @default.
- W2406816195 hasConcept C104317684 @default.
- W2406816195 hasConcept C144647389 @default.
- W2406816195 hasConcept C149232098 @default.
- W2406816195 hasConcept C153911025 @default.
- W2406816195 hasConcept C167625842 @default.
- W2406816195 hasConcept C181199279 @default.
- W2406816195 hasConcept C185592680 @default.
- W2406816195 hasConcept C41625074 @default.
- W2406816195 hasConcept C43617362 @default.
- W2406816195 hasConcept C55493867 @default.
- W2406816195 hasConcept C85265743 @default.
- W2406816195 hasConcept C86803240 @default.
- W2406816195 hasConcept C87933860 @default.
- W2406816195 hasConceptScore W2406816195C10010492 @default.
- W2406816195 hasConceptScore W2406816195C101660505 @default.
- W2406816195 hasConceptScore W2406816195C104317684 @default.
- W2406816195 hasConceptScore W2406816195C144647389 @default.
- W2406816195 hasConceptScore W2406816195C149232098 @default.
- W2406816195 hasConceptScore W2406816195C153911025 @default.
- W2406816195 hasConceptScore W2406816195C167625842 @default.
- W2406816195 hasConceptScore W2406816195C181199279 @default.
- W2406816195 hasConceptScore W2406816195C185592680 @default.
- W2406816195 hasConceptScore W2406816195C41625074 @default.
- W2406816195 hasConceptScore W2406816195C43617362 @default.
- W2406816195 hasConceptScore W2406816195C55493867 @default.
- W2406816195 hasConceptScore W2406816195C85265743 @default.
- W2406816195 hasConceptScore W2406816195C86803240 @default.
- W2406816195 hasConceptScore W2406816195C87933860 @default.
- W2406816195 hasLocation W24068161951 @default.
- W2406816195 hasLocation W24068161952 @default.
- W2406816195 hasOpenAccess W2406816195 @default.
- W2406816195 hasPrimaryLocation W24068161951 @default.
- W2406816195 hasRelatedWork W1967346650 @default.
- W2406816195 hasRelatedWork W1969938465 @default.
- W2406816195 hasRelatedWork W1990222541 @default.
- W2406816195 hasRelatedWork W2005187240 @default.
- W2406816195 hasRelatedWork W2006950854 @default.
- W2406816195 hasRelatedWork W2031721803 @default.
- W2406816195 hasRelatedWork W2033159596 @default.
- W2406816195 hasRelatedWork W2072397813 @default.
- W2406816195 hasRelatedWork W2082948633 @default.
- W2406816195 hasRelatedWork W2085160186 @default.
- W2406816195 hasRelatedWork W2141084973 @default.
- W2406816195 hasRelatedWork W2159351303 @default.
- W2406816195 hasRelatedWork W223448283 @default.
- W2406816195 hasRelatedWork W2235453479 @default.
- W2406816195 hasRelatedWork W2292429748 @default.
- W2406816195 hasRelatedWork W2410172738 @default.
- W2406816195 hasRelatedWork W2469457019 @default.
- W2406816195 hasRelatedWork W2470133457 @default.
- W2406816195 hasRelatedWork W2908462029 @default.
- W2406816195 hasRelatedWork W54105219 @default.
- W2406816195 isParatext "false" @default.
- W2406816195 isRetracted "false" @default.
- W2406816195 magId "2406816195" @default.
- W2406816195 workType "book-chapter" @default.