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- W2406880011 abstract "The relative position identification of some of the proteins in human blood plasma and serum after separation by two-dimensional electrophoresis (2-DE) in gel slabs have been studied. Antibodies specific to the protein to be identified were first immobilized on Protein A-Sepharose. Serum or plasma samples devoid of this protein were obtained following adsorption and subsequently the protein itself was eluted. The preparations were examined by 2-DE, silverstaining and blotting with parallel analysis of the purified proteins. Position identification in 2-DE gels of the spots of alpha 1-microglobulin (= protein HC) is shown as an example. The technique has general applicability not only for identification of the positions in 2-DE gels of serum and plasma proteins but also for proteins in other fluids, tissues and organs from humans and other species." @default.
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- W2406880011 date "1991-01-01" @default.
- W2406880011 modified "2023-09-25" @default.
- W2406880011 title "Identification of human blood plasma and serum proteins in two-dimensional gels by use of protein A-Sepharose: application to alpha 1-microglobulin." @default.
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