FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2407140280> ?p ?o ?g. }

• W2407140280 endingPage "4583" @default.
• W2407140280 startingPage "4570" @default.
• W2407140280 abstract "The yeast Pichia pastoris is a widely used host for recombinant protein production. Understanding its physiology at extremely low growth rates is a first step in the direction of decoupling product formation from cellular growth and therefore of biotechnological relevance. Retentostat cultivation is an excellent tool for studying microbes at extremely low specific growth rates but has so far not been implemented for P. pastoris Retentostat feeding regimes were based on the maintenance energy requirement (mS) and maximum biomass yield on glucose (YX /S (max)) estimated from steady-state glucose-limited chemostat cultures. Aerobic retentostat cultivation enabled reproducible, smooth transitions from a specific growth rate (μ) of 0.025 h(-1) to near-zero specific growth rates (μ < 0.001 h(-1)). At these near-zero specific growth rates, viability remained at least 97%. The value of mS at near-zero growth rates was 3.1 ± 0.1 mg glucose per g biomass and h, which was 3-fold lower than the mS estimated from faster-growing chemostat cultures. This difference indicated that P. pastoris reduces its maintenance energy requirement at extremely low μ, a phenomenon not previously observed in eukaryotes. Intracellular levels of glycogen and trehalose increased, while μ progressively declined during retentostat cultivation. Transcriptional reprogramming toward zero growth included the upregulation of many transcription factors as well as stress-related genes and the downregulation of cell cycle genes. This study underlines the relevance of comparative analysis of maintenance energy metabolism, which has an important impact on large-scale industrial processes.The yeast Pichia pastoris naturally lives on trees and can utilize different carbon sources, among them glucose, glycerol, and methanol. In biotechnology, it is widely used for the production of recombinant proteins. For both the understanding of life in its natural habitat and optimized production processes, a better understanding of cell physiology at an extremely low growth rate would be of extraordinary value. Therefore, we have grown P. pastoris in a retentostat, which allows the cultivation of metabolically active cells even at zero growth. Here we reached doubling times as long as 38 days and found that P. pastoris decreases its maintenance energy demand 3-fold during very slow growth, which enables it to survive with a much lower substrate supply than baker's yeast." @default.
• W2407140280 created "2016-06-24" @default.
• W2407140280 creator A5038319556 @default.
• W2407140280 creator A5046986424 @default.
• W2407140280 creator A5050115274 @default.
• W2407140280 creator A5054535009 @default.
• W2407140280 creator A5066968633 @default.
• W2407140280 creator A5070213705 @default.
• W2407140280 creator A5087000945 @default.
• W2407140280 date "2016-08-01" @default.
• W2407140280 modified "2023-10-16" @default.
• W2407140280 title "Pichia pastoris Exhibits High Viability and a Low Maintenance Energy Requirement at Near-Zero Specific Growth Rates" @default.
• W2407140280 cites W1037853884 @default.
• W2407140280 cites W1533533640 @default.
• W2407140280 cites W1558671359 @default.
• W2407140280 cites W1574136604 @default.
• W2407140280 cites W1702609754 @default.
• W2407140280 cites W1873692790 @default.
• W2407140280 cites W1967620272 @default.
• W2407140280 cites W1969353942 @default.
• W2407140280 cites W1973327968 @default.
• W2407140280 cites W1975446929 @default.
• W2407140280 cites W1975952108 @default.
• W2407140280 cites W1977237360 @default.
• W2407140280 cites W1977501098 @default.
• W2407140280 cites W1981020054 @default.
• W2407140280 cites W1982968657 @default.
• W2407140280 cites W1986364415 @default.
• W2407140280 cites W1986488367 @default.
• W2407140280 cites W1992207353 @default.
• W2407140280 cites W1992299163 @default.
• W2407140280 cites W2005619516 @default.
• W2407140280 cites W2007786225 @default.
• W2407140280 cites W2008141632 @default.
• W2407140280 cites W2011778636 @default.
• W2407140280 cites W2013459567 @default.
• W2407140280 cites W2016050838 @default.
• W2407140280 cites W2028230342 @default.
• W2407140280 cites W2029705967 @default.
• W2407140280 cites W2031049841 @default.
• W2407140280 cites W2033006928 @default.
• W2407140280 cites W2044272062 @default.
• W2407140280 cites W2046437093 @default.
• W2407140280 cites W2046594775 @default.
• W2407140280 cites W2052078471 @default.
• W2407140280 cites W2053803408 @default.
• W2407140280 cites W2059639708 @default.
• W2407140280 cites W2061058657 @default.
• W2407140280 cites W2067092006 @default.
• W2407140280 cites W2073138847 @default.
• W2407140280 cites W2080428447 @default.
• W2407140280 cites W2088685767 @default.
• W2407140280 cites W2088691956 @default.
• W2407140280 cites W2098107306 @default.
• W2407140280 cites W2101442503 @default.
• W2407140280 cites W2107632694 @default.
• W2407140280 cites W2111436190 @default.
• W2407140280 cites W2112736223 @default.
• W2407140280 cites W2113070980 @default.
• W2407140280 cites W2124039604 @default.
• W2407140280 cites W2124327251 @default.
• W2407140280 cites W2125610369 @default.
• W2407140280 cites W2128361384 @default.
• W2407140280 cites W2134766732 @default.
• W2407140280 cites W2137262144 @default.
• W2407140280 cites W2137801078 @default.
• W2407140280 cites W2147901684 @default.
• W2407140280 cites W2155333588 @default.
• W2407140280 cites W2155638586 @default.
• W2407140280 cites W2157009707 @default.
• W2407140280 cites W2158180844 @default.
• W2407140280 cites W2164216268 @default.
• W2407140280 cites W2166866734 @default.
• W2407140280 cites W2167812532 @default.
• W2407140280 cites W2170160990 @default.
• W2407140280 cites W2170984819 @default.
• W2407140280 cites W2189427509 @default.
• W2407140280 cites W2435084741 @default.
• W2407140280 cites W2885987207 @default.
• W2407140280 cites W2917973694 @default.
• W2407140280 cites W829478918 @default.
• W2407140280 doi "https://doi.org/10.1128/aem.00638-16" @default.
• W2407140280 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4984280" @default.
• W2407140280 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27208115" @default.
• W2407140280 hasPublicationYear "2016" @default.
• W2407140280 type Work @default.
• W2407140280 sameAs 2407140280 @default.
• W2407140280 citedByCount "42" @default.
• W2407140280 countsByYear W24071402802017 @default.
• W2407140280 countsByYear W24071402802018 @default.
• W2407140280 countsByYear W24071402802019 @default.
• W2407140280 countsByYear W24071402802020 @default.
• W2407140280 countsByYear W24071402802021 @default.
• W2407140280 countsByYear W24071402802022 @default.
• W2407140280 countsByYear W24071402802023 @default.
• W2407140280 crossrefType "journal-article" @default.
• W2407140280 hasAuthorship W2407140280A5038319556 @default.
• W2407140280 hasAuthorship W2407140280A5046986424 @default.

• next