Matches in SemOpenAlex for { <https://semopenalex.org/work/W2407192762> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2407192762 endingPage "15" @default.
- W2407192762 startingPage "106" @default.
- W2407192762 abstract "17-beta-Hydroxysteroid dehydrogenase was purified from human placenta. The purification process included the following steps: 50% saturated ammonium sulfate precipitation; heat treatment; affinity column chromatography; and preparative SDS gel electrophoretic elution. This enzyme was also characterized by HPLC gel filtration and two dimensional gel electrophoresis with isoelectrofocusing. The results indicate that the molecular weight of these enzymes in their native condition is around 68 kDa and 34 kDa in SDS PAGE. Therefore, the active form of the enzyme is an identical dimmer in nature. There are three charged isomers as demonstrated by isoelectrofocusing. The antiserum against the 17-beta-HSD was induced by injection of purified human placenta 17-beta-HSD in rabbits. The antiserum was collected and characterized by ELISA, immunohistochemistry, immunoblot and immunoprecipitation tests. The results showed that the antibody titer was over 1:12,800, and specificity against human placenta 17-beta-HSD was also observed." @default.
- W2407192762 created "2016-06-24" @default.
- W2407192762 creator A5010925700 @default.
- W2407192762 creator A5068666288 @default.
- W2407192762 creator A5077377702 @default.
- W2407192762 date "1993-07-01" @default.
- W2407192762 modified "2023-09-23" @default.
- W2407192762 title "The induction and characterization of antiserum against human placenta 17-beta-hydroxysteroid dehydrogenase." @default.
- W2407192762 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8290653" @default.
- W2407192762 hasPublicationYear "1993" @default.
- W2407192762 type Work @default.
- W2407192762 sameAs 2407192762 @default.
- W2407192762 citedByCount "0" @default.
- W2407192762 crossrefType "journal-article" @default.
- W2407192762 hasAuthorship W2407192762A5010925700 @default.
- W2407192762 hasAuthorship W2407192762A5068666288 @default.
- W2407192762 hasAuthorship W2407192762A5077377702 @default.
- W2407192762 hasConcept C104317684 @default.
- W2407192762 hasConcept C116084860 @default.
- W2407192762 hasConcept C153911025 @default.
- W2407192762 hasConcept C159654299 @default.
- W2407192762 hasConcept C172680121 @default.
- W2407192762 hasConcept C179247698 @default.
- W2407192762 hasConcept C181199279 @default.
- W2407192762 hasConcept C185592680 @default.
- W2407192762 hasConcept C193363816 @default.
- W2407192762 hasConcept C203014093 @default.
- W2407192762 hasConcept C2776317432 @default.
- W2407192762 hasConcept C2776428949 @default.
- W2407192762 hasConcept C2776953305 @default.
- W2407192762 hasConcept C2779234561 @default.
- W2407192762 hasConcept C43617362 @default.
- W2407192762 hasConcept C54355233 @default.
- W2407192762 hasConcept C55493867 @default.
- W2407192762 hasConcept C71829478 @default.
- W2407192762 hasConcept C86803240 @default.
- W2407192762 hasConcept C91523082 @default.
- W2407192762 hasConceptScore W2407192762C104317684 @default.
- W2407192762 hasConceptScore W2407192762C116084860 @default.
- W2407192762 hasConceptScore W2407192762C153911025 @default.
- W2407192762 hasConceptScore W2407192762C159654299 @default.
- W2407192762 hasConceptScore W2407192762C172680121 @default.
- W2407192762 hasConceptScore W2407192762C179247698 @default.
- W2407192762 hasConceptScore W2407192762C181199279 @default.
- W2407192762 hasConceptScore W2407192762C185592680 @default.
- W2407192762 hasConceptScore W2407192762C193363816 @default.
- W2407192762 hasConceptScore W2407192762C203014093 @default.
- W2407192762 hasConceptScore W2407192762C2776317432 @default.
- W2407192762 hasConceptScore W2407192762C2776428949 @default.
- W2407192762 hasConceptScore W2407192762C2776953305 @default.
- W2407192762 hasConceptScore W2407192762C2779234561 @default.
- W2407192762 hasConceptScore W2407192762C43617362 @default.
- W2407192762 hasConceptScore W2407192762C54355233 @default.
- W2407192762 hasConceptScore W2407192762C55493867 @default.
- W2407192762 hasConceptScore W2407192762C71829478 @default.
- W2407192762 hasConceptScore W2407192762C86803240 @default.
- W2407192762 hasConceptScore W2407192762C91523082 @default.
- W2407192762 hasIssue "3" @default.
- W2407192762 hasLocation W24071927621 @default.
- W2407192762 hasOpenAccess W2407192762 @default.
- W2407192762 hasPrimaryLocation W24071927621 @default.
- W2407192762 hasRelatedWork W1521506765 @default.
- W2407192762 hasRelatedWork W1522047977 @default.
- W2407192762 hasRelatedWork W1966188665 @default.
- W2407192762 hasRelatedWork W2001016396 @default.
- W2407192762 hasRelatedWork W2014617056 @default.
- W2407192762 hasRelatedWork W2110193451 @default.
- W2407192762 hasRelatedWork W2115357251 @default.
- W2407192762 hasRelatedWork W2116224626 @default.
- W2407192762 hasRelatedWork W2406759195 @default.
- W2407192762 hasRelatedWork W2407192762 @default.
- W2407192762 hasVolume "17" @default.
- W2407192762 isParatext "false" @default.
- W2407192762 isRetracted "false" @default.
- W2407192762 magId "2407192762" @default.
- W2407192762 workType "article" @default.