FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2407486977> ?p ?o ?g. }

• W2407486977 endingPage "2268" @default.
• W2407486977 startingPage "2255" @default.
• W2407486977 abstract "We previously demonstrated that the activated leukocyte cell adhesion molecule (ALCAM/CD166) can interact with galectin-8 (Gal-8) in endothelial cells. ALCAM is a member of the immunoglobulin superfamily that promotes homophilic and heterophilic cell-cell interactions. Gal-8 is a tandem-repeat-type galectin, known as a matricellular protein involved in cell adhesion. Here, we analyzed the physical interaction between both molecules in breast cancer cells and the functional relevance of this phenomenon.We performed binding assays by surface plasmon resonance to study the interaction between Gal-8 and the recombinant glycosylated ALCAM ectodomain or endogenous ALCAM from MDA-MB-231 breast cancer cells. We also analyzed the binding of ALCAM-silenced or control breast cancer cells to immobilized Gal-8 by SPR. In internalization assays, we evaluated the influence of Gal-8 on ALCAM surface localization.We showed that recombinant glycosylated ALCAM and endogenous ALCAM from breast carcinoma cells physically interacted with Gal-8 in a glycosylation-dependent fashion displaying a differential behavior compared to non-glycosylated ALCAM. Moreover, ALCAM-silenced breast cancer cells exhibited reduced binding to Gal-8 relative to control cells. Importantly, exogenously added Gal-8 provoked ALCAM segregation, probably trapping this adhesion molecule at the surface of breast cancer cells.Our data indicate that Gal-8 interacts with ALCAM at the surface of breast cancer cells through glycosylation-dependent mechanisms.A novel heterophilic interaction between ALCAM and Gal-8 is demonstrated here, suggesting its physiologic relevance in the biology of breast cancer cells." @default.
• W2407486977 created "2016-06-24" @default.
• W2407486977 creator A5009000446 @default.
• W2407486977 creator A5010409382 @default.
• W2407486977 creator A5010753532 @default.
• W2407486977 creator A5010952197 @default.
• W2407486977 creator A5015469384 @default.
• W2407486977 creator A5016346855 @default.
• W2407486977 creator A5022067713 @default.
• W2407486977 creator A5024226262 @default.
• W2407486977 creator A5024682572 @default.
• W2407486977 creator A5029926740 @default.
• W2407486977 creator A5040301543 @default.
• W2407486977 creator A5059969100 @default.
• W2407486977 creator A5074901091 @default.
• W2407486977 creator A5076209427 @default.
• W2407486977 creator A5079519652 @default.
• W2407486977 creator A5079985016 @default.
• W2407486977 date "2016-10-01" @default.
• W2407486977 modified "2023-10-14" @default.
• W2407486977 title "Glycosylation-dependent binding of galectin-8 to activated leukocyte cell adhesion molecule (ALCAM/CD166) promotes its surface segregation on breast cancer cells" @default.
• W2407486977 cites W1525058971 @default.
• W2407486977 cites W1787109533 @default.
• W2407486977 cites W1811352252 @default.
• W2407486977 cites W1912614720 @default.
• W2407486977 cites W1964035033 @default.
• W2407486977 cites W1965644953 @default.
• W2407486977 cites W1967347349 @default.
• W2407486977 cites W1968014420 @default.
• W2407486977 cites W1974003580 @default.
• W2407486977 cites W1975372137 @default.
• W2407486977 cites W1980035883 @default.
• W2407486977 cites W1981612562 @default.
• W2407486977 cites W1981954000 @default.
• W2407486977 cites W1984890062 @default.
• W2407486977 cites W1985679384 @default.
• W2407486977 cites W1993838215 @default.
• W2407486977 cites W1996326203 @default.
• W2407486977 cites W2000630911 @default.
• W2407486977 cites W2003945230 @default.
• W2407486977 cites W2006132448 @default.
• W2407486977 cites W2007440106 @default.
• W2407486977 cites W2015049954 @default.
• W2407486977 cites W2015493647 @default.
• W2407486977 cites W2017107299 @default.
• W2407486977 cites W2017544831 @default.
• W2407486977 cites W2020917997 @default.
• W2407486977 cites W2022050193 @default.
• W2407486977 cites W2025178368 @default.
• W2407486977 cites W2033691898 @default.
• W2407486977 cites W2035469701 @default.
• W2407486977 cites W2037011184 @default.
• W2407486977 cites W2037821908 @default.
• W2407486977 cites W2038361233 @default.
• W2407486977 cites W2045622596 @default.
• W2407486977 cites W2053023842 @default.
• W2407486977 cites W2055015884 @default.
• W2407486977 cites W2062541805 @default.
• W2407486977 cites W2066545420 @default.
• W2407486977 cites W2076894591 @default.
• W2407486977 cites W2079766592 @default.
• W2407486977 cites W2080432978 @default.
• W2407486977 cites W2084642021 @default.
• W2407486977 cites W2088373848 @default.
• W2407486977 cites W2091524596 @default.
• W2407486977 cites W2092485298 @default.
• W2407486977 cites W2101979288 @default.
• W2407486977 cites W2106428582 @default.
• W2407486977 cites W2107806009 @default.
• W2407486977 cites W2113702155 @default.
• W2407486977 cites W2115346329 @default.
• W2407486977 cites W2116413866 @default.
• W2407486977 cites W2118300139 @default.
• W2407486977 cites W2128695497 @default.
• W2407486977 cites W2129049215 @default.
• W2407486977 cites W2131879353 @default.
• W2407486977 cites W2132102722 @default.
• W2407486977 cites W2134443544 @default.
• W2407486977 cites W2134976204 @default.
• W2407486977 cites W2137857595 @default.
• W2407486977 cites W2139773613 @default.
• W2407486977 cites W2140915719 @default.
• W2407486977 cites W2141767234 @default.
• W2407486977 cites W2150525787 @default.
• W2407486977 cites W2151411303 @default.
• W2407486977 cites W2156941611 @default.
• W2407486977 cites W2158620400 @default.
• W2407486977 cites W2159365185 @default.
• W2407486977 cites W2188713841 @default.
• W2407486977 cites W2214000001 @default.
• W2407486977 cites W2281637108 @default.
• W2407486977 cites W4247832335 @default.
• W2407486977 doi "https://doi.org/10.1016/j.bbagen.2016.04.019" @default.
• W2407486977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27130882" @default.
• W2407486977 hasPublicationYear "2016" @default.
• W2407486977 type Work @default.
• W2407486977 sameAs 2407486977 @default.
• W2407486977 citedByCount "22" @default.

• next