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• W2407524625 abstract "Abstract Biosynthesis and molecular structure of major histocompatibility complex (MHC) class II antigens of DR2/DR7 hairy cells were analyzed by two-dimensional polyacrylamide-gel electrophoresis (2D-PAGE). Two anti- human Ia monoclonal antibodies (mAb) were used to immunoprecipitate DR and DR-linked DC/DS molecules. Monoclonal antibody VI 15 C recognizes DR (I-E-like) molecules and CA 2.06 precipitates DR and DR-linked DC/DS (I-A-like) molecules in DR7 allotypes. Studies were performed on a pure population of hairy cells before and after culture with phorbol ester: 12-O-tetradecanoyl phorbol-13-acetate (TPA), 5 azacytidine (5 Aza), sodium butyrate (NA-BU), and phytohemagglutinin (PHA-P). Before any treatment, hairy cells expressed and synthesized DR antigens: DR alpha and beta subunits appeared both qualitatively and quantitatively normal by 2D-PAGE profile. In contrast, the hairy cells failed to express and synthesized any DC/DS molecule. The lack of DC/DS molecular expression was restored after culture in presence of TPA, sodium butyrate, and 5 azacytidine, but not after PHA-P treatment. Differential molecular expression of MHC class II antigens in leukemic cells provides a model to define further discrete stages of hemopoietic differentiation and study the role of these molecules in the cellular interactions occurring during differentiation." @default.
• W2407524625 created "2016-06-24" @default.
• W2407524625 creator A5033258369 @default.
• W2407524625 creator A5053956114 @default.
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• W2407524625 date "1984-07-01" @default.
• W2407524625 modified "2023-09-30" @default.
• W2407524625 title "Differential expression of HLA-DR and HLA-DC/DS molecules in a patient with hairy cell leukemia: restoration of HLA-DC/DS expression by (12-0- tetradecanoyl phorbol-13-acetate), 5 azacytidine, and sodium butyrate" @default.
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• W2407524625 doi "https://doi.org/10.1182/blood.v64.1.33.33" @default.
• W2407524625 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6203577" @default.
• W2407524625 hasPublicationYear "1984" @default.
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