FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2407538439> ?p ?o ?g. }

• W2407538439 endingPage "239" @default.
• W2407538439 startingPage "229" @default.
• W2407538439 abstract "We previously found that homocysteine (Hcy) induced plasma membrane flip-flop, apoptosis, and necrosis in cardiomyocytes. Inactivation of flippase by Hcy induced membrane flip-flop, while apoptosis was induced via a NOX2-dependent mechanism. It has been suggested that S-adenosylhomocysteine (SAH) is the main causative factor in hyperhomocysteinemia (HHC)-induced pathogenesis of cardiovascular disease. Therefore, we evaluated whether the observed cytotoxic effect of Hcy in cardiomyocytes is SAH dependent. Rat cardiomyoblasts (H9c2 cells) were treated under different conditions: (1) non-treated control (1.5 nM intracellular SAH with 2.8 μM extracellular l-Hcy), (2) incubation with 50 μM adenosine-2,3-dialdehyde (ADA resulting in 83.5 nM intracellular SAH, and 1.6 μM extracellular l-Hcy), (3) incubation with 2.5 mM d,l-Hcy (resulting in 68 nM intracellular SAH and 1513 μM extracellular l-Hcy) with or without 10 μM reactive oxygen species (ROS)-inhibitor apocynin, and (4) incubation with 100 nM, 10 μM, and 100 μM SAH. We then determined the effect on annexin V/propodium iodide positivity, flippase activity, caspase-3 activity, intracellular NOX2 and p47phox expression and localization, and nuclear ROS production. In contrast to Hcy, ADA did not induce apoptosis, necrosis, or membrane flip-flop. Remarkably, both ADA and Hcy induced a significant increase in nuclear NOX2 expression. However, in contrast to ADA, Hcy additionally induced nuclear p47phox expression, increased nuclear ROS production, and inactivated flippase. Incubation with SAH did not have an effect on cell viability, nor on flippase activity, nor on nuclear NOX2-, p47phox expression or nuclear ROS production. HHC-induced membrane flip-flop and apoptosis in cardiomyocytes is due to increased Hcy levels and not primarily related to increased intracellular SAH, which plays a crucial role in nuclear p47phox translocation and subsequent ROS production." @default.
• W2407538439 created "2016-06-24" @default.
• W2407538439 creator A5004518590 @default.
• W2407538439 creator A5004809273 @default.
• W2407538439 creator A5011287788 @default.
• W2407538439 creator A5027056106 @default.
• W2407538439 creator A5036128395 @default.
• W2407538439 creator A5053613000 @default.
• W2407538439 creator A5058133438 @default.
• W2407538439 creator A5065784242 @default.
• W2407538439 creator A5083354719 @default.
• W2407538439 creator A5088953887 @default.
• W2407538439 creator A5090997097 @default.
• W2407538439 date "2011-07-08" @default.
• W2407538439 modified "2023-09-27" @default.
• W2407538439 title "Homocysteine-induced cardiomyocyte apoptosis and plasma membrane flip-flop are independent of S-adenosylhomocysteine: a crucial role for nuclear p47phox" @default.
• W2407538439 cites W1539883814 @default.
• W2407538439 cites W1590509970 @default.
• W2407538439 cites W1935061717 @default.
• W2407538439 cites W1977377731 @default.
• W2407538439 cites W1981512601 @default.
• W2407538439 cites W1983889201 @default.
• W2407538439 cites W1985678955 @default.
• W2407538439 cites W19867578 @default.
• W2407538439 cites W1987226612 @default.
• W2407538439 cites W1992205845 @default.
• W2407538439 cites W2009151436 @default.
• W2407538439 cites W2017704247 @default.
• W2407538439 cites W2033706395 @default.
• W2407538439 cites W2043108620 @default.
• W2407538439 cites W2051146938 @default.
• W2407538439 cites W2082452850 @default.
• W2407538439 cites W2093200537 @default.
• W2407538439 cites W2095109205 @default.
• W2407538439 cites W2099136646 @default.
• W2407538439 cites W2111925756 @default.
• W2407538439 cites W2120124457 @default.
• W2407538439 cites W2122070162 @default.
• W2407538439 cites W2132470039 @default.
• W2407538439 cites W2136573376 @default.
• W2407538439 cites W2138532578 @default.
• W2407538439 cites W2138952800 @default.
• W2407538439 cites W2139835631 @default.
• W2407538439 cites W2140418591 @default.
• W2407538439 cites W2152813651 @default.
• W2407538439 cites W2157561127 @default.
• W2407538439 cites W2160189772 @default.
• W2407538439 cites W2162350450 @default.
• W2407538439 cites W2166998646 @default.
• W2407538439 cites W2172011253 @default.
• W2407538439 cites W4229539731 @default.
• W2407538439 doi "https://doi.org/10.1007/s11010-011-0973-4" @default.
• W2407538439 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21739151" @default.
• W2407538439 hasPublicationYear "2011" @default.
• W2407538439 type Work @default.
• W2407538439 sameAs 2407538439 @default.
• W2407538439 citedByCount "12" @default.
• W2407538439 countsByYear W24075384392012 @default.
• W2407538439 countsByYear W24075384392014 @default.
• W2407538439 countsByYear W24075384392015 @default.
• W2407538439 countsByYear W24075384392018 @default.
• W2407538439 countsByYear W24075384392019 @default.
• W2407538439 countsByYear W24075384392021 @default.
• W2407538439 crossrefType "journal-article" @default.
• W2407538439 hasAuthorship W2407538439A5004518590 @default.
• W2407538439 hasAuthorship W2407538439A5004809273 @default.
• W2407538439 hasAuthorship W2407538439A5011287788 @default.
• W2407538439 hasAuthorship W2407538439A5027056106 @default.
• W2407538439 hasAuthorship W2407538439A5036128395 @default.
• W2407538439 hasAuthorship W2407538439A5053613000 @default.
• W2407538439 hasAuthorship W2407538439A5058133438 @default.
• W2407538439 hasAuthorship W2407538439A5065784242 @default.
• W2407538439 hasAuthorship W2407538439A5083354719 @default.
• W2407538439 hasAuthorship W2407538439A5088953887 @default.
• W2407538439 hasAuthorship W2407538439A5090997097 @default.
• W2407538439 hasBestOaLocation W24075384392 @default.
• W2407538439 hasConcept C185592680 @default.
• W2407538439 hasConcept C190283241 @default.
• W2407538439 hasConcept C2776523773 @default.
• W2407538439 hasConcept C2777090595 @default.
• W2407538439 hasConcept C2777209548 @default.
• W2407538439 hasConcept C2778918659 @default.
• W2407538439 hasConcept C2779637612 @default.
• W2407538439 hasConcept C2779719074 @default.
• W2407538439 hasConcept C28406088 @default.
• W2407538439 hasConcept C41625074 @default.
• W2407538439 hasConcept C48349386 @default.
• W2407538439 hasConcept C55493867 @default.
• W2407538439 hasConcept C79879829 @default.
• W2407538439 hasConcept C86803240 @default.
• W2407538439 hasConcept C95444343 @default.
• W2407538439 hasConceptScore W2407538439C185592680 @default.
• W2407538439 hasConceptScore W2407538439C190283241 @default.
• W2407538439 hasConceptScore W2407538439C2776523773 @default.
• W2407538439 hasConceptScore W2407538439C2777090595 @default.
• W2407538439 hasConceptScore W2407538439C2777209548 @default.
• W2407538439 hasConceptScore W2407538439C2778918659 @default.
• W2407538439 hasConceptScore W2407538439C2779637612 @default.

• next