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- W2408028309 abstract "To investigate the effects of various concentrations of all-trans retinoic acid (RA) on the rat Müller cells in vitro.A rat Müller cell line was used in this study. Rat Müller cells were cultured with varying levels of RA for 24 h to 48 h. We examined cellular morphology under phase contrast microscope, cell proliferation using MTT assay, viable cell numbers by hemocytometer counting and cell apoptosis with flow cytometry.At lower concentration (< 0.1 microM), RA didn't change the cell's appearance and didn't inhibit the cell proliferation (P > 0.05). However, at higher concentration (1 microM, 10 microM, 100 microM), RA could inhibit the proliferation of Müller cells accompanied with morphological changes in a time- and dose-dependent manner. Furthermore, different concentration of RA (5 microM, 10 microM, 20 microM) could induce cell apoptosis. When using 20 microM RA to treat cells for 48 h, a significant decrease in cell numbers and an obvious increment of apoptotic cells were observed. The percentage of apoptotic cell was (35.87 +/- 7.40)% (P < 0.01, vs. control).RA can inhibit the proliferation and induce the apoptosis of müller cells in a time- and dose-dependent manner." @default.
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- W2408028309 date "2007-09-01" @default.
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- W2408028309 title "[Effects of all-trans retinoic acid on the rat Müller cell in vitro]." @default.
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