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• W2408045571 abstract "ObjectiveTo investigate the effect of gene silencing of integrin β1 on the proliferation and secretory function of airway smooth muscle cells(ASMC) in asthmatic mice.MethodsIntegrin β1 gene was silenced by using RNAi technology in the fifth generation ASMC of normal and asthmatic mice. The integrin β1 mRNA expression and integrin β1 protein were analyzed by RT-PCR and Western blot respectively before and after the integrin β1 gene silencing in ASMC. The cell proliferation changes of ASMC were measured by MTT assay before and after gene silencing of integrin β1.The cell cycle distribution and apoptosis rate were analyzed by flow cytometry. The expression of interleukin (IL)-6 and RANTES were analyzed by ELISA assay.Results(1)Integrin β1 mRNA expression by RT-PCR (0.907±0.041) was significantly higher in asthma control group compared with normal control group (0.527±0.027) (t=24.632, P 0.05). (2) Integrin β1 protein expression (0.733±0.067) was significantly higher(t=13.622, P 0.05). (3) The absorbance value was significantly enhanced in the asthma control group compared with the same period of normal control group(t=9.528, 5.799, 3.372, all P 0.05). (4) The S+ G2/M phase ratio of ASMC was significantly higher in asthma control group[(49±4)%] compared with normal control group (34±4)% (t=8.035, P 0.05). (5) The apoptosis rate was significantly lower in asthma control group [(3.9±1.4)%] compared with normal control group [(7.4±0.5)%] (t=7.465, P 0.05). (6) The secreted IL-6 [(545±28)ng/L] and RANTES [(345±28)ng/L] were higher in asthma control group compared with the normal control group [(219±26) ng/L, (138±16)ng/L, respectively, t=26.789, 20.451, all P<0.05], and was significantly decreased in the asthma siRNA intervention group [(347±26)ng/L, (250±24)ng/L] compared with asthma control group(t=6.192, 4.590, all P<0.05), while there was no significant difference in the normal control group after the intervention.ConclusionGene silencing targeting integrin β1 inhibited proliferation and secretion, but promoted apoptosis of ASMC from asthmatic mice.Key words: Asthma; Antigen, CD29; Myocytes, smooth muscle" @default.
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• W2408045571 date "2010-11-01" @default.
• W2408045571 modified "2023-09-24" @default.
• W2408045571 title "Effect of gene silencing of integrin β1 on the proliferation and secretory function of airway smooth muscle in asthmatic mice" @default.
• W2408045571 doi "https://doi.org/10.3760/cma.j.issn.1001-0939.2010.11.003" @default.
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