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- W2408113638 abstract "The effect of induction temperature on fermentation parameters has been investigated extensively using Escherichia coli M5248[pNKM21], a producer of recombinant human interleukin-2 (rhIL-2). In this recombinant microorganism, the gene expression of rhIL-2 is regulated by the cI857 repressor and promoter system. The recombinant fermentation parameters studied in this work include the cell growth, protein synthesis, cell viability, plasmid stability, -lactamase activity, and rhIL-2 productivity. Interrelationships of such fermentation parameters have been analyzed through a quantitative assessment of the experimental data set obtained at eight different culture conditions. While the expression of rhIL-2 gene was repressed at culture temperatures below with little effect on other fermentation parameters, under the conditions of rhIL-2 production $>(36~44^circ{C})$ the cell growth, plasmid stability, and -lactamase activity were, as induction temperature was increased, more profoundly reduced. Although the rhIL-2 content in the insoluble protein fraction was maximum at , total rhIL-2 production in the culture volume was found to be highest at the induction temperature of . This was in contrast to the previously known optimum induction temperature of the P promoter system $>(40~42^circ{C})$.Explanations for such a discrepancy have been proposed based on a product formation kinetics, and their implications have been discussed in detail.l." @default.
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- W2408113638 date "1992-01-01" @default.
- W2408113638 modified "2023-09-23" @default.
- W2408113638 title "Effect of Induction Temperature on the $P_L$ Promoter Controlled Production of Recombinant Human Interleukin-2 in Escherichia coli" @default.
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