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- W2408917696 abstract "AbstractThe polymerase chain reaction (PCR) provides a rapid method for generating a large amount of a defined region of DNA without recourse to cloning. However, direct sequencing of this amplified material is tedious, time-consuming, and frequently generates large amounts of normal sequence data. Based on the PCR technique, a number of screening methods for detecting DNA mutations have been developed and a number of these are described in this volume. Many of these techniques are dependent on the formation of heteroduplexes during the amplification reaction, i.e., mixed double-stranded DNA molecules in which one strand is derived from the normal allele and the other from the mutant or altered allele. The heteroduplexes can exhibit a variety of changes in their physical properties and can be readily detected by a variety of techniques. In the many cases, such heteroduplexes can be detected without the use of radiolabeling.KeywordsPolymerase Chain Reaction ProductPolymerase Chain Reaction TechniqueAmmonium PersulphateBulldog ClipSaran WrapThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves." @default.
- W2408917696 created "2016-06-24" @default.
- W2408917696 creator A5037536965 @default.
- W2408917696 date "2003-11-14" @default.
- W2408917696 modified "2023-10-18" @default.
- W2408917696 title "Screening for Mutations in the Human Antithrombin Gene by Hydrolink D-5000™ and MDE™ Gel Electrophoresis" @default.
- W2408917696 cites W2075800046 @default.
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- W2408917696 doi "https://doi.org/10.1385/1-59259-248-1:125" @default.
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