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- W2409055320 abstract "Gene sequencing and haemolysis of bacteria in Clarias gariepinus post juveniles sampled fromAjilete location on Yewa River, Ogun state, Nigeria was carried out. Bacteria were isolated from thegut, gills and skin of the fish. Thirty bacteria isolates were selected and the DNAs were extractedusing hexadecyltrimethylammonium bromide (CTAB) method and Polymerase Chain Reaction(PCR) amplification of the isolates was carried out using universal primer for bacteria. Thepurification of PCR product was done using ethanol precipitation and thereafter the DNAs weresequenced using automated DNA sequencer. The sequence data were analyzed on the GenBankdatabase (NCBI) using Basic Local Alignment Search Tool (BLAST) to find the nearest relatedsequence, and to construct the phylogenetic relationship. The haemolysis of the selected isolateswere also carried out. Data collected were subjected to descriptive statistics. Nine genera of bacteria isolated were Escherichia, Staphylococcus, Enterobacter, Proteus, Pseudomonas,Klebsiella, Citrobacter, Streptococcus and Bacillus. The 16S rRNA gene sequencing methodidentified the isolates as strains of Brevibacillus agri, Bacillus cereus, Ignatzschineria indica,Comamonas kerstersii, Proteus vulgaris, Lysinibacillus sphaericus, Proteus hauseri, Clostridiumbifermentans, Proteus mirabilis, Pseudomonas putida, Escherichia coli, Comamonas jiangduensis,Enterobacter cloacae, Serratia marcescens, Alcaligenes faecalis, Lysinibacillus fusiformis,Myroides odoratimimus. Haemolysis showed that M. odoratimimus, B. cereus, C. kerstersii,P. vulgaris, C. bifermentans, E. coli and E. cloacae all had β-haemolysis. A. faecalis displayed α-haemolysis while, B. agri, I. indica, L. sphaericus, P. hauseri, L. fusiformis, S. marcescens werenon-haemolytic. This study confirms the reliability of the 16S rRNA gene sequencing method. Thisstudy also concludes that there are different pathogenic bacteria species that are associated withC. gariepinus." @default.
- W2409055320 created "2016-06-24" @default.
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- W2409055320 date "2016-01-10" @default.
- W2409055320 modified "2023-09-27" @default.
- W2409055320 title "Gene Sequencing and Haemolysis of Bacteria in Clarias gariepinus From Ajilete Location on Yewa River" @default.
- W2409055320 doi "https://doi.org/10.9734/arrb/2016/26923" @default.
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