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- W2409649842 abstract "AIM:To explore the effects of recombinant Balillus Calmette-Guerin secreting IL-12 on the development of spleen T cell subsets of neonatal BALB/c mice.METHODS:Twenty-four neonatal BALB/c mices were divided 3 groups:Control,BCG,r-BCG groups,which inoculated with BCG and r-BCG intraperiotoneally within 2-3 days after birth.Four weeks later,spleen cells of mice were isolated and the percentage of CD3+CD8+IFN-γ+/CD3+CD8-IFN-γ+/CD3+CD8+IL-4+/CD3+CD8-IL-4+/CD4+Foxp3+ T cells,which represent Tc1/Th1/Tc2/Th2/Treg cells respectively were detected by flow cytometry at single cells level,and Th1/Th2,Tc1/Tc2 ratio were calculated.RESULTS:The percentages of Th1,Tc1 cell of BCG-vaccinated mice,r-BCG-vaccinated mice were significant higher than control mice(P0.01),and there was no difference between the two vaccinated groups.The ratio of Th1/Th2,Tc1/Tc2 and total IFN-γ/IL-4 of two vaccinated groups were higher than control group.The percentages of Treg cells were no difference among the three groups(P0.05).CONCLUSION:Neonatal BCG and r-BCG-vaccination can significantly increase Tc1 and Th1 cells and Th1/Th2,Tc1/Tc2 ratio in spleen cells.Neonatal BCG and r-BCG vaccination may have an same effects on development of T cell subsets in neonatal BALB/c mice.The percentage of CD4+Foxp3+ T cells among three groups were no significant difference." @default.
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- W2409649842 date "2008-06-01" @default.
- W2409649842 modified "2023-09-27" @default.
- W2409649842 title "[Effects of recombinant Balillus Calmette-Guerin secreting IL-12 vaccination on development of T cell subsets in neonatal BALB/c mice]." @default.
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