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• W2409788809 abstract "The UV-VIS spectroscopy and fluorescence spectroscopy were applied to investigate the mechanism of the interaction between methylene blue (MB) and herring sperm DNA by using acridine orange as a spectral probe in Tris-HCl buffer (pH 7.0). The chief action mode was comfirmed to be an intercalation binding at a lower MB concentration but the mixed action modes at a higher concentration. Combining ratio of the binary complex is n(MB) : n(DNA)= 10 : 1, the binding constant of MB with DNA is K-26 degrees C(circle minus)=2.46 X 10(5) L center dot mol(-1) and the apparent molor absorptivity is epsilon = 5.70 X 10(6) L center dot mol(-1)center dot cm(-1). Meanwhile, the interaction of MB with DNA was effected by the changes of pH and temperature. The results deduce that the interaction between MB and DNA is an enthalpy driven reaction." @default.
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• W2409788809 date "2008-02-28" @default.
• W2409788809 modified "2023-09-23" @default.
• W2409788809 title "Spectroscopy Studies of Interaction between Methylene Blue and Herring Sperm DNA" @default.
• W2409788809 hasPublicationYear "2008" @default.
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