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• W2409802018 abstract "Finding protein evidence (PE) for protein coding genes is a primary task of the Phase I Chromosome-Centric Human Proteome Project (C-HPP). Currently, there are 2948 PE level 2-4 coding genes per neXtProt, which are deemed missing proteins in the human proteome. As most samples prepared and analyzed in the C-HPP framework were focusing on detergent soluble proteins, we posit that as a natural composition the cytoplasmic detergent-insoluble proteins (DIPs) represent a source of finding missing proteins. We optimized a workflow and separated cytoplasmic DIPs from three human lung and three human hepatoma cell lines via differential speed centrifugation. We verified that the detergent-soluble proteins (DSPs) could be sufficiently depleted and the cytoplasmic DIP isolation was partially reproducible with Spearman r > 0.70 according to two independent SILAC MS experiments. Through label-free MS, we identified 4524 and 4156 DIPs from lung and liver cells, respectively. Among them, a total of 23 missing proteins (22 PE2 and 1 PE4) were identified by MS, and 18 of them had translation evidence; in addition, six PE5 proteins were identified by MS, three with translation evidence. We showed that cytoplasmic DIPs were not an enrichment of transmembrane proteins and were chromosome-, cell type-, and tissue-specific. Furthermore, we demonstrated that DIPs were distinct from DSPs in terms of structural and physical-chemical features. In conclusion, we have found 23 missing proteins and 6 PE5 proteins from the cytoplasmic insoluble proteome that is biologically and physical-chemically different from the soluble proteome, suggesting that cytoplasmic DIPs carry comprehensive and valuable information for finding PE of missing proteins. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the data set identifier PXD001694." @default.
• W2409802018 created "2016-06-24" @default.
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• W2409802018 date "2015-07-10" @default.
• W2409802018 modified "2023-09-26" @default.
• W2409802018 title "Identification of Missing Proteins Defined by Chromosome-Centric Proteome Project in the Cytoplasmic Detergent-Insoluble Proteins" @default.
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• W2409802018 cites W1966168891 @default.
• W2409802018 cites W1966733718 @default.
• W2409802018 cites W1969615465 @default.
• W2409802018 cites W1970577444 @default.
• W2409802018 cites W1978456286 @default.
• W2409802018 cites W1978985034 @default.
• W2409802018 cites W1979748052 @default.
• W2409802018 cites W1980599684 @default.
• W2409802018 cites W1981963802 @default.
• W2409802018 cites W1984952667 @default.
• W2409802018 cites W1991334711 @default.
• W2409802018 cites W1996098588 @default.
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• W2409802018 cites W2013429682 @default.
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• W2409802018 cites W2027818319 @default.
• W2409802018 cites W2032416004 @default.
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• W2409802018 cites W2072772620 @default.
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• W2409802018 doi "https://doi.org/10.1021/pr501103r" @default.
• W2409802018 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26108252" @default.
• W2409802018 hasPublicationYear "2015" @default.
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