FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2409962489> ?p ?o ?g. }

• W2409962489 endingPage "e127" @default.
• W2409962489 startingPage "e127" @default.
• W2409962489 abstract "Objective: Ageing is the biggest independent risk factor for cardiovascular disease; the leading cause of death worldwide. Recent studies demonstrate that age-related endothelial dysfunction, a major risk factor for cardiovascular disease, may be due to decreased bioavailability of the endogenous vasodilator nitric oxide (NO), synthesised in the vascular endothelium by endothelial nitric oxide synthase (eNOS); which is phosphorylated and thus activated by AMP-activated protein kinase (AMPK). Vascular reactivity is further regulated via the perivascular adipose tissue (PVAT), which has a net anti-contractile effect. PVAT is known to be a source of additional NO, as well as secreting factors that augment local endothelial NO production, potentially through AMPK activation. Whilst this anti-contractile effect of PVAT is well characterised in young animals, it is unknown what effects ageing has on this relationship. Our hypothesis is that PVAT dysfunction may occur with ageing. Design and method: Small diameter mesenteric arteries were taken from male Wistar rats aged 3 months old (m.o.), 12m.o., 18m.o. and 24m.o. and contractility to U46619 (10nM-3 μM) and phenylephrine (1nM-30 μM) assessed via wire-myography in the presence and absence of PVAT and the NO-synthesis inhibitor L-NNA (50 μM). Western blotting for AMPK, p-AMPK, eNOS and p-eNOS was performed on mesenteric artery samples from rats aged 3m.o. and 24m.o. Results: Results showed that PVAT was anti-contractile in 3m.o. and 12m.o. old rats but that this effect was lost by 18m.o., remaining absent at 24m.o.. Incubation with L-NNA reversed the anti-contractile effect of PVAT at 3m.o., but not 24m.o. Expression of total AMPK was reduced in arteries from 24m.o. compared to 3m.o. whereas the ratio of p-AMPK/total AMPK remained unchanged. Expression of eNOS remained unchanged at 24m.o., whereas p-eNOS/total eNOS ratio was significantly decreased. Conclusions: The anti-contractile effect of PVAT is lost with age in rats, due at least in part to reduced NO bioavailability. This reduced bioavailability may be the result of reduced eNOS phosphorylation downstream of reduced AMPK expression." @default.
• W2409962489 created "2016-06-24" @default.
• W2409962489 creator A5000754946 @default.
• W2409962489 creator A5056967745 @default.
• W2409962489 creator A5090299015 @default.
• W2409962489 creator A5090981933 @default.
• W2409962489 date "2015-06-01" @default.
• W2409962489 modified "2023-10-14" @default.
• W2409962489 title "LB03.05" @default.
• W2409962489 doi "https://doi.org/10.1097/01.hjh.0000467695.36876.d8" @default.
• W2409962489 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26102731" @default.
• W2409962489 hasPublicationYear "2015" @default.
• W2409962489 type Work @default.
• W2409962489 sameAs 2409962489 @default.
• W2409962489 citedByCount "1" @default.
• W2409962489 countsByYear W24099624892018 @default.
• W2409962489 crossrefType "journal-article" @default.
• W2409962489 hasAuthorship W2409962489A5000754946 @default.
• W2409962489 hasAuthorship W2409962489A5056967745 @default.
• W2409962489 hasAuthorship W2409962489A5090299015 @default.
• W2409962489 hasAuthorship W2409962489A5090981933 @default.
• W2409962489 hasBestOaLocation W24099624891 @default.
• W2409962489 hasConcept C120770815 @default.
• W2409962489 hasConcept C126322002 @default.
• W2409962489 hasConcept C134018914 @default.
• W2409962489 hasConcept C171089720 @default.
• W2409962489 hasConcept C184235292 @default.
• W2409962489 hasConcept C1918360 @default.
• W2409962489 hasConcept C2776820930 @default.
• W2409962489 hasConcept C2777622882 @default.
• W2409962489 hasConcept C2777952589 @default.
• W2409962489 hasConcept C2778326061 @default.
• W2409962489 hasConcept C2780124434 @default.
• W2409962489 hasConcept C2780771799 @default.
• W2409962489 hasConcept C2780972559 @default.
• W2409962489 hasConcept C39133596 @default.
• W2409962489 hasConcept C500499127 @default.
• W2409962489 hasConcept C519581460 @default.
• W2409962489 hasConcept C55493867 @default.
• W2409962489 hasConcept C71924100 @default.
• W2409962489 hasConcept C84393581 @default.
• W2409962489 hasConcept C86803240 @default.
• W2409962489 hasConcept C97029542 @default.
• W2409962489 hasConceptScore W2409962489C120770815 @default.
• W2409962489 hasConceptScore W2409962489C126322002 @default.
• W2409962489 hasConceptScore W2409962489C134018914 @default.
• W2409962489 hasConceptScore W2409962489C171089720 @default.
• W2409962489 hasConceptScore W2409962489C184235292 @default.
• W2409962489 hasConceptScore W2409962489C1918360 @default.
• W2409962489 hasConceptScore W2409962489C2776820930 @default.
• W2409962489 hasConceptScore W2409962489C2777622882 @default.
• W2409962489 hasConceptScore W2409962489C2777952589 @default.
• W2409962489 hasConceptScore W2409962489C2778326061 @default.
• W2409962489 hasConceptScore W2409962489C2780124434 @default.
• W2409962489 hasConceptScore W2409962489C2780771799 @default.
• W2409962489 hasConceptScore W2409962489C2780972559 @default.
• W2409962489 hasConceptScore W2409962489C39133596 @default.
• W2409962489 hasConceptScore W2409962489C500499127 @default.
• W2409962489 hasConceptScore W2409962489C519581460 @default.
• W2409962489 hasConceptScore W2409962489C55493867 @default.
• W2409962489 hasConceptScore W2409962489C71924100 @default.
• W2409962489 hasConceptScore W2409962489C84393581 @default.
• W2409962489 hasConceptScore W2409962489C86803240 @default.
• W2409962489 hasConceptScore W2409962489C97029542 @default.
• W2409962489 hasIssue "Supplement 1" @default.
• W2409962489 hasLocation W24099624891 @default.
• W2409962489 hasOpenAccess W2409962489 @default.
• W2409962489 hasPrimaryLocation W24099624891 @default.
• W2409962489 hasRelatedWork W2063444986 @default.
• W2409962489 hasRelatedWork W2078516115 @default.
• W2409962489 hasRelatedWork W2097716096 @default.
• W2409962489 hasRelatedWork W2106490387 @default.
• W2409962489 hasRelatedWork W2543544500 @default.
• W2409962489 hasRelatedWork W2580258152 @default.
• W2409962489 hasRelatedWork W2898713340 @default.
• W2409962489 hasRelatedWork W3176409244 @default.
• W2409962489 hasRelatedWork W4311255504 @default.
• W2409962489 hasRelatedWork W4378648562 @default.
• W2409962489 hasVolume "33" @default.
• W2409962489 isParatext "false" @default.
• W2409962489 isRetracted "false" @default.
• W2409962489 magId "2409962489" @default.
• W2409962489 workType "article" @default.