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- W2410003735 abstract "To produce large quantities of primary cell suspensions on efficient technique is described which is named Biomix-Method. By this technique first tissue mince is performed automatically using tissue amounts between 300 g and 500 g. Trypsinization takes place in a three-litre trypsinization vessel using a stirring mechanism according to a defined trypsinization mode. In regard to optimal cell seeding concentration and a justifiable productivity one kidney of newborn or suckling calf yields 6--9 1 cell suspension and that of piglet 1,8-2,3 1, respectively. Proliferation behaviour and effectivity of primary cells are discussed and compared with other cell systems." @default.
- W2410003735 created "2016-06-24" @default.
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- W2410003735 date "1979-01-01" @default.
- W2410003735 modified "2023-09-23" @default.
- W2410003735 title "[Production of primary cell cultures on a semi-industrial scale. 1. Modification of the trypsinization procedure--possible means to improve cell yield]." @default.
- W2410003735 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/547911" @default.
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