Matches in SemOpenAlex for { <https://semopenalex.org/work/W2410036577> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2410036577 endingPage "981" @default.
- W2410036577 startingPage "981" @default.
- W2410036577 abstract "Purpose: To evaluate the anti-apoptotic effect of phyllanthin on alcohol-induced liver cell death in HepG2 cells alone and in co-culture with human monocytic (THP-1) differentiated macrophage cells.Methods: Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cells were pretreated with 1, 5 and 10 μM phyllanthin for 24 h followed by 1300 mM alcohol for HepG2 cells and 2000 mM alcohol for the co-cultured cells. Thereafter, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP) changes, apoptotic cell death and caspase-3/7 activities were assessed.Results: Alcohol exposure significantly increased intracellular ROS generation (p < 0.001), decreased MMP changes (p < 0.001), increased the number of apoptotic and necrotic cells (p < 0.001) and also induced higher caspase-3/7 activity (p < 0.001) in the co-culture with THP-1 differentiated macrophage cells than in HepG2 cells alone. Pretreatment of HepG2 cells and co-cultured cells with phyllanthin for 24 h prior to alcohol exposure significantly decreased intracellular production of ROS (p < 0.001) and also increased the change in MMP (p < 0.001) as well as caused a decrease in the number of apoptotic and necrotic cells (p < 0.001), but inhibited caspase-3/7 activity (p < 0.001).Conclusion: The results indicate that phyllanthin treatment may have a significant therapeutic effect on alcohol-related liver diseases.Keywords: Hepatoprotective, Liver diseases, Human monocytic cells, Reactive oxygen species, Apoptosis, Co-culture, Mitochondrial membrane potential" @default.
- W2410036577 created "2016-06-24" @default.
- W2410036577 creator A5018082833 @default.
- W2410036577 creator A5026186660 @default.
- W2410036577 creator A5053078672 @default.
- W2410036577 date "2016-05-30" @default.
- W2410036577 modified "2023-09-28" @default.
- W2410036577 title "Anti-apoptotic effects of phyllanthin against alcoholinduced liver cell death" @default.
- W2410036577 cites W144263607 @default.
- W2410036577 cites W1541649582 @default.
- W2410036577 cites W1966601836 @default.
- W2410036577 cites W1973376874 @default.
- W2410036577 cites W1990071637 @default.
- W2410036577 cites W2002096942 @default.
- W2410036577 cites W2004981017 @default.
- W2410036577 cites W2008954179 @default.
- W2410036577 cites W2009835065 @default.
- W2410036577 cites W2022877915 @default.
- W2410036577 cites W2027328852 @default.
- W2410036577 cites W2042812804 @default.
- W2410036577 cites W2044975783 @default.
- W2410036577 cites W2053542703 @default.
- W2410036577 cites W2068762746 @default.
- W2410036577 cites W2070549269 @default.
- W2410036577 cites W2073925856 @default.
- W2410036577 cites W2082339006 @default.
- W2410036577 cites W2085757386 @default.
- W2410036577 cites W2091440199 @default.
- W2410036577 cites W2089314840 @default.
- W2410036577 doi "https://doi.org/10.4314/tjpr.v15i5.12" @default.
- W2410036577 hasPublicationYear "2016" @default.
- W2410036577 type Work @default.
- W2410036577 sameAs 2410036577 @default.
- W2410036577 citedByCount "3" @default.
- W2410036577 countsByYear W24100365772020 @default.
- W2410036577 countsByYear W24100365772021 @default.
- W2410036577 countsByYear W24100365772023 @default.
- W2410036577 crossrefType "journal-article" @default.
- W2410036577 hasAuthorship W2410036577A5018082833 @default.
- W2410036577 hasAuthorship W2410036577A5026186660 @default.
- W2410036577 hasAuthorship W2410036577A5053078672 @default.
- W2410036577 hasBestOaLocation W24100365771 @default.
- W2410036577 hasConcept C153911025 @default.
- W2410036577 hasConcept C185592680 @default.
- W2410036577 hasConcept C190283241 @default.
- W2410036577 hasConcept C2780783641 @default.
- W2410036577 hasConcept C31573885 @default.
- W2410036577 hasConcept C48349386 @default.
- W2410036577 hasConcept C53227056 @default.
- W2410036577 hasConcept C54355233 @default.
- W2410036577 hasConcept C55493867 @default.
- W2410036577 hasConcept C79879829 @default.
- W2410036577 hasConcept C81885089 @default.
- W2410036577 hasConcept C86803240 @default.
- W2410036577 hasConcept C98274493 @default.
- W2410036577 hasConceptScore W2410036577C153911025 @default.
- W2410036577 hasConceptScore W2410036577C185592680 @default.
- W2410036577 hasConceptScore W2410036577C190283241 @default.
- W2410036577 hasConceptScore W2410036577C2780783641 @default.
- W2410036577 hasConceptScore W2410036577C31573885 @default.
- W2410036577 hasConceptScore W2410036577C48349386 @default.
- W2410036577 hasConceptScore W2410036577C53227056 @default.
- W2410036577 hasConceptScore W2410036577C54355233 @default.
- W2410036577 hasConceptScore W2410036577C55493867 @default.
- W2410036577 hasConceptScore W2410036577C79879829 @default.
- W2410036577 hasConceptScore W2410036577C81885089 @default.
- W2410036577 hasConceptScore W2410036577C86803240 @default.
- W2410036577 hasConceptScore W2410036577C98274493 @default.
- W2410036577 hasIssue "5" @default.
- W2410036577 hasLocation W24100365771 @default.
- W2410036577 hasLocation W24100365772 @default.
- W2410036577 hasOpenAccess W2410036577 @default.
- W2410036577 hasPrimaryLocation W24100365771 @default.
- W2410036577 hasRelatedWork W1988995391 @default.
- W2410036577 hasRelatedWork W2166261174 @default.
- W2410036577 hasRelatedWork W2368058280 @default.
- W2410036577 hasRelatedWork W2377878450 @default.
- W2410036577 hasRelatedWork W2390892693 @default.
- W2410036577 hasRelatedWork W2410350978 @default.
- W2410036577 hasRelatedWork W2472820331 @default.
- W2410036577 hasRelatedWork W2727689036 @default.
- W2410036577 hasRelatedWork W4223916243 @default.
- W2410036577 hasRelatedWork W4307179377 @default.
- W2410036577 hasVolume "15" @default.
- W2410036577 isParatext "false" @default.
- W2410036577 isRetracted "false" @default.
- W2410036577 magId "2410036577" @default.
- W2410036577 workType "article" @default.