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- W2410106639 endingPage "430" @default.
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- W2410106639 abstract "Intermediate filaments (IFs) are involved in multiple cellular processes that are essential for the maintenance of cell and tissue integrity. To achieve this crucial function, IFs have to be organized as long and resistant filaments across the cells and to be tightly anchored at the cell periphery. This anchoring takes place at the level desmosomes and hemidesmosomes through proteins belonging to the spectraplakin family. Here, we focus on the sole nematode Caenorhabditis elegans spectraplakin locus vab-10 that is essential to connect the epidermis to the cuticle apically and to the muscles basally. After briefly reviewing the structure of the gene, we first present the genetic tools available to study this gene as well as the reagents to examine the distribution of its translation products. We discuss the functional assays that enable examining their function. Finally, we detail a genetic method to identify spectraplakin functional partners through RNAi screens, and a biochemical method to examine the phosphorylation status of IFs." @default.
- W2410106639 created "2016-06-24" @default.
- W2410106639 creator A5030538355 @default.
- W2410106639 creator A5039084091 @default.
- W2410106639 creator A5043043816 @default.
- W2410106639 date "2016-01-01" @default.
- W2410106639 modified "2023-09-27" @default.
- W2410106639 title "Functional and Genetic Analysis of VAB-10 Spectraplakin in Caenorhabditis elegans" @default.
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- W2410106639 doi "https://doi.org/10.1016/bs.mie.2015.05.005" @default.
- W2410106639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26778569" @default.
- W2410106639 hasPublicationYear "2016" @default.
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