Matches in SemOpenAlex for { <https://semopenalex.org/work/W2410247943> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2410247943 endingPage "133" @default.
- W2410247943 startingPage "125" @default.
- W2410247943 abstract "Summary Mice genetically deficient in factor VIII (fVIII) are a model of hemophilia A. As a first step to reproduce in this mouse model what occurs over time in hemophilia A patients treated with human fVIII (hfVIII), we have investigated the time course and the characteristics of their immune response to hfVIII, after multiple intravenous injections. Anti-hfVIII antibodies appeared after four to five injections. They were IgG1 and to a lesser extent IgG2, indicating that they were induced by both Th2 and Th1 cells. Inhibitors appeared after six injections. CD4+ enriched splenocytes from hfVIII-treated mice proliferated in response to fVIII and secreted IL-10: in a few mice they secreted also IFN-γ and in one mouse IL-4, but never IL-2. A hfVIII-specific T cell line derived from hfVIII-treated mice secreted both IL-4 and IFN-γ, suggesting that it included both Th1 and Th2 cells. CD4+ enriched splenocytes of hfVIII-treated mice recognized all hfVIII domains. Thus, hemophilic mice develop an immune response to hfVIII administered intravenously similar to that of hemophilia A patients. Their anti-hfVIII antibodies can be inhibitors and belong to IgG subclasses homologous to those of inhibitors in hemophilic patients; their anti-hfVIII CD4+ cells recognize a complex repertoire and both Th1 and Th2 cytokines, and especially IL-10, may drive the antibody synthesis. Abbreviations used: antibodies, Ab; antigen presenting cells, APC; Arbitrary Units, AU; enzyme-linked immunosorbant assay, ELISA; factor VIII, fVIII; human factor VIII, hf VIII; intravenous, i.v.; optical density, OD; polymerase chain reaction, PCR; phosphate buffered saline solution, PBS; PBS containing 3% bovine serum albumin, PBS/BSA; PBS containing 0.05% polyoxyethylene sorbitan monolaurate, PBS/Tween-20; phytohemoagglutinin, PHA; stimulation index, SI" @default.
- W2410247943 created "2016-06-24" @default.
- W2410247943 creator A5008146377 @default.
- W2410247943 creator A5013248904 @default.
- W2410247943 creator A5036111632 @default.
- W2410247943 creator A5042163133 @default.
- W2410247943 creator A5053387578 @default.
- W2410247943 creator A5073547682 @default.
- W2410247943 creator A5075242123 @default.
- W2410247943 creator A5080114064 @default.
- W2410247943 date "2001-01-01" @default.
- W2410247943 modified "2023-10-13" @default.
- W2410247943 title "Mechanism of the Immune Response to Human Factor VIII in Murine Hemophilia A" @default.
- W2410247943 doi "https://doi.org/10.1055/s-0037-1612915" @default.
- W2410247943 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11204564" @default.
- W2410247943 hasPublicationYear "2001" @default.
- W2410247943 type Work @default.
- W2410247943 sameAs 2410247943 @default.
- W2410247943 citedByCount "75" @default.
- W2410247943 countsByYear W24102479432012 @default.
- W2410247943 countsByYear W24102479432013 @default.
- W2410247943 countsByYear W24102479432014 @default.
- W2410247943 countsByYear W24102479432015 @default.
- W2410247943 countsByYear W24102479432016 @default.
- W2410247943 countsByYear W24102479432017 @default.
- W2410247943 countsByYear W24102479432020 @default.
- W2410247943 countsByYear W24102479432022 @default.
- W2410247943 countsByYear W24102479432023 @default.
- W2410247943 crossrefType "journal-article" @default.
- W2410247943 hasAuthorship W2410247943A5008146377 @default.
- W2410247943 hasAuthorship W2410247943A5013248904 @default.
- W2410247943 hasAuthorship W2410247943A5036111632 @default.
- W2410247943 hasAuthorship W2410247943A5042163133 @default.
- W2410247943 hasAuthorship W2410247943A5053387578 @default.
- W2410247943 hasAuthorship W2410247943A5073547682 @default.
- W2410247943 hasAuthorship W2410247943A5075242123 @default.
- W2410247943 hasAuthorship W2410247943A5080114064 @default.
- W2410247943 hasConcept C147483822 @default.
- W2410247943 hasConcept C159047783 @default.
- W2410247943 hasConcept C159654299 @default.
- W2410247943 hasConcept C189976730 @default.
- W2410247943 hasConcept C203014093 @default.
- W2410247943 hasConcept C71924100 @default.
- W2410247943 hasConcept C8891405 @default.
- W2410247943 hasConceptScore W2410247943C147483822 @default.
- W2410247943 hasConceptScore W2410247943C159047783 @default.
- W2410247943 hasConceptScore W2410247943C159654299 @default.
- W2410247943 hasConceptScore W2410247943C189976730 @default.
- W2410247943 hasConceptScore W2410247943C203014093 @default.
- W2410247943 hasConceptScore W2410247943C71924100 @default.
- W2410247943 hasConceptScore W2410247943C8891405 @default.
- W2410247943 hasFunder F4320306086 @default.
- W2410247943 hasIssue "01" @default.
- W2410247943 hasLocation W24102479431 @default.
- W2410247943 hasLocation W24102479432 @default.
- W2410247943 hasOpenAccess W2410247943 @default.
- W2410247943 hasPrimaryLocation W24102479431 @default.
- W2410247943 hasRelatedWork W1484759019 @default.
- W2410247943 hasRelatedWork W2006139140 @default.
- W2410247943 hasRelatedWork W2040191638 @default.
- W2410247943 hasRelatedWork W2184033775 @default.
- W2410247943 hasRelatedWork W2220102944 @default.
- W2410247943 hasRelatedWork W2327402772 @default.
- W2410247943 hasRelatedWork W2404822265 @default.
- W2410247943 hasRelatedWork W2409441623 @default.
- W2410247943 hasRelatedWork W2515380456 @default.
- W2410247943 hasRelatedWork W4210976760 @default.
- W2410247943 hasVolume "85" @default.
- W2410247943 isParatext "false" @default.
- W2410247943 isRetracted "false" @default.
- W2410247943 magId "2410247943" @default.
- W2410247943 workType "article" @default.