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- W2410345148 abstract "To prepare monoclonal antibodies (McAbs) against human programmed death-1 ligant 1(PD-L1) and identify its bioactivity.We immunized the BALB/c mice with Thioredoxin-(PD-L1) recombination protein which expressed by prokaryotic system. Prepare hybridoma cell by hybridoma technology and used enzyme-linked immunosorbent assay(ELISA) and Western-blotting assays to select positive hybridoma identify cell. Competition inhibition ELISA was carried out to identify the special bioactivity of antibody.4 hybridoma cell strains which could secrete anti-(PD-L1) antibodies stably were selected. The McAbs has good affinity with its receptor. Purify anti-(PD-L1) with title 1:32 000 was obtained after large quantity preparation. At the same time we obtained 1 cell stain which could secret special anti-Trx McAbs.We obtained anti-(PD-L1) McAbs with good bioactivity successfully, which lay the foundation for further study." @default.
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- W2410345148 date "2010-10-01" @default.
- W2410345148 modified "2023-09-27" @default.
- W2410345148 title "[Preparation and identification of the monoclonal antibodies against programmed death-1 ligant 1]." @default.
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