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- W2410366760 abstract "Abstract A method is described for extracting tissue samples with hexane-acetone and hydrolyzing the fat and protein with aqueous sodium hydroxide. p,p’-DDT and its metabolites are measured on a gas chromatograph with an electron capture detector after cleanup on an alumina column. The new cleanup procedure gives 98% recoveries for p,p’-DDT and its metabolites added to fat in vitro, compared with 84% recovery by a modified commonly used method. Comparison by Student's f-test of the new method with the modified method on 32 cattle biopsy, 46 bird, and 20 fish samples for in vivo incurred p,p’-DDT residues showed that the results by the new method were significantly higher at the 95% confidence level. Alkali-hydrolyzed extract did not contaminate the detector. The new procedure avoids the use of dimethylformamide and shortens the time of analyses. A correlation (r=0.68) was observed between the fat-soluble protein concentration of the n-hexane extract and the p,p’-DDT residues." @default.
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- W2410366760 date "1979-09-01" @default.
- W2410366760 modified "2023-09-27" @default.
- W2410366760 title "Cleanup of Biological Samples for Determining p,p’-DDT and Its Metabolites" @default.
- W2410366760 doi "https://doi.org/10.1093/jaoac/62.5.1150" @default.
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