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• W2410529100 abstract "During the United States Department of Agriculture (USDA) National Animal Health Monitoring System's (NAHMS) 2007-2008 beef study, 567 producers from 24 US States were offered the opportunity to collect fecal samples from weaned beef calves and have them evaluated for the presence of parasite eggs (Phase 1). Participating producers were provided with instructions and materials for sample collection. Up to 20 fresh fecal samples were collected from each of the 99 participating operations. Fresh fecal samples were submitted to one of 3 randomly assigned laboratories for evaluation. Upon arrival at the laboratories, all samples were processed for the enumeration of strongyle, Nematodirus, and Trichuris eggs using the modified Wisconsin technique. The presence or absence of coccidian oocysts and tapeworm eggs was also noted. In submissions where the strongyle eggs per gram exceeded 30, aliquots from 2 to 6 animals were pooled for DNA extraction. Extracted DNA was subjected to genus level polymerase chain reaction (PCR) identification for the presence of Ostertagia, Cooperia, Haemonchus, Oesophagostomum, and Trichostrongylus. In this study, 85.6% of the samples had strongyle type, Nematodirus, and Trichuris eggs. Among the samples evaluated, 91% had Cooperia, 79% Ostertagia, 53% Haemonchus, 38% Oesophagostomum, 18% Nematodirus, 7% Trichuris, and 3% Trichostrongylus. The prevalence of coccidia and tapeworm eggs was 59.9% and 13.7%, respectively. Pendant l’étude de 2007–2008 chez les bovins effectuée par le Système national de surveillance des maladies animales (NAHMS) du Département de l’agriculture des États-Unis (USDA), 567 producteurs provenant de 24 états américains se sont vus offrir l’opportunité de prélever des échantillons de fèces de veaux sevrés et de les faire analyser pour la présence d’oeufs de parasite (Phase 1). On a fourni aux producteurs participants les instructions et le matériel pour le prélèvement d’échantillon. Jusqu’à 20 échantillons de fèces fraiches furent prélevés de chacune des 99 opérations participantes. Les échantillons de fèces fraiches furent soumis de manière aléatoire pour évaluation à l’un des trois laboratoires participants. Suite à l’arrivée au laboratoire, tous les échantillons étaient traités pour énumération des strongles, de Nematodirus, et d’oeufs de Trichuris en utilisant la technique de Wisconsin modifiée. La présence ou l’absence d’ookystes de coccidie et d’oeufs de vers plats furent également notées. Dans les échantillons soumis et dont le nombre d’oeufs de strongles par gramme dépassait 30, des aliquots de 2 à 6 animaux étaient regroupés pour extraction de l’ADN. L’ADN extrait était soumis à une réaction d’amplification en chaine par la polymérase (PCR) pour une identification au genre de la présence d’Ostertagia, de Cooperia, d’Haemonchus, d’Oesophagostomum, et de Trichostrongylus. Dans la présente étude, 85,6 % des échantillons avaient des strongles, du Nematodirus, et des oeufs de Trichuris. Parmi les échantillons évalués, 91 % avaient du Cooperia, 79 % de l’Ostertagia, 53 % de l’Haemoncus, 38 % de l’Oesophagostomum, 18 % du Nematodirus, 7 % du Trichuris, et 3 % du Trichostrongylus. Les prévalences de coccidies et d’oeufs de vers plats étaient respectivement de 59,9 % et 13,7 %.(Traduit par Docteur Serge Messier)." @default.
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• W2410529100 date "2015-10-01" @default.
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• W2410529100 title "Prevalence of internal parasites in beef cows in the United States: Results of the National Animal Health Monitoring System's (NAHMS) beef study, 2007-2008." @default.
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