FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2410641965> ?p ?o ?g. }

• W2410641965 endingPage "2215" @default.
• W2410641965 startingPage "2206" @default.
• W2410641965 abstract "Potent and selective recognition and modulation of disease-relevant RNAs remain a daunting challenge. We previously examined the utility of the U1A N-terminal RNA recognition motif as a scaffold for tailoring new RNA hairpin recognition and showed that as few as one or two mutations can result in moderate affinity (low μM dissociation constant) for the human immunodeficiency virus (HIV) trans-activation response element (TAR) RNA, an RNA hairpin controlling transcription of the human immunodeficiency virus (HIV) genome. Here, we use yeast display and saturation mutagenesis of established RNA-binding regions in U1A to identify new synthetic proteins that potently and selectively bind TAR RNA. Our best candidate has truly altered, not simply broadened, RNA-binding selectivity; it binds TAR with subnanomolar affinity (apparent dissociation constant of ∼0.5 nM) but does not appreciably bind the original U1A RNA target (U1hpII). It specifically recognizes the TAR RNA hairpin in the context of the HIV-1 5′-untranslated region, inhibits the interaction between TAR RNA and an HIV trans-activator of transcription (Tat)-derived peptide, and suppresses Tat/TAR-dependent transcription. Proteins described in this work are among the tightest TAR RNA-binding reagents–small molecule, nucleic acid, or protein–reported to date and thus have potential utility as therapeutics and basic research tools. Moreover, our findings demonstrate how a naturally occurring RNA recognition motif can be dramatically resurfaced through mutation, leading to potent and selective recognition—and modulation—of disease-relevant RNA." @default.
• W2410641965 created "2016-06-24" @default.
• W2410641965 creator A5000107010 @default.
• W2410641965 creator A5003287208 @default.
• W2410641965 creator A5022867480 @default.
• W2410641965 creator A5030075929 @default.
• W2410641965 creator A5071886170 @default.
• W2410641965 creator A5080450473 @default.
• W2410641965 creator A5087529327 @default.
• W2410641965 date "2016-06-13" @default.
• W2410641965 modified "2023-09-25" @default.
• W2410641965 title "An Evolved RNA Recognition Motif That Suppresses HIV-1 Tat/TAR-Dependent Transcription" @default.
• W2410641965 cites W1485536019 @default.
• W2410641965 cites W1528632786 @default.
• W2410641965 cites W1582827974 @default.
• W2410641965 cites W1583466614 @default.
• W2410641965 cites W1588341390 @default.
• W2410641965 cites W1964455506 @default.
• W2410641965 cites W1971592398 @default.
• W2410641965 cites W1973460506 @default.
• W2410641965 cites W1976306943 @default.
• W2410641965 cites W1977889638 @default.
• W2410641965 cites W1978127515 @default.
• W2410641965 cites W1983404383 @default.
• W2410641965 cites W1984106312 @default.
• W2410641965 cites W1985278607 @default.
• W2410641965 cites W1986262235 @default.
• W2410641965 cites W1987117140 @default.
• W2410641965 cites W1988283896 @default.
• W2410641965 cites W1990246245 @default.
• W2410641965 cites W1990789777 @default.
• W2410641965 cites W1993926172 @default.
• W2410641965 cites W1997956959 @default.
• W2410641965 cites W1998664265 @default.
• W2410641965 cites W1999269806 @default.
• W2410641965 cites W2005369655 @default.
• W2410641965 cites W2010801138 @default.
• W2410641965 cites W2014599804 @default.
• W2410641965 cites W2019518442 @default.
• W2410641965 cites W2020193594 @default.
• W2410641965 cites W2024408347 @default.
• W2410641965 cites W2029077569 @default.
• W2410641965 cites W2034414337 @default.
• W2410641965 cites W2035516686 @default.
• W2410641965 cites W2041751293 @default.
• W2410641965 cites W2042750467 @default.
• W2410641965 cites W2042891181 @default.
• W2410641965 cites W2043548965 @default.
• W2410641965 cites W2053716543 @default.
• W2410641965 cites W2053861881 @default.
• W2410641965 cites W2068254744 @default.
• W2410641965 cites W2070777085 @default.
• W2410641965 cites W2071908003 @default.
• W2410641965 cites W2075498752 @default.
• W2410641965 cites W2085065303 @default.
• W2410641965 cites W2086205453 @default.
• W2410641965 cites W2087744052 @default.
• W2410641965 cites W2088201490 @default.
• W2410641965 cites W2090025697 @default.
• W2410641965 cites W2092915328 @default.
• W2410641965 cites W2108699324 @default.
• W2410641965 cites W2111308603 @default.
• W2410641965 cites W2113525941 @default.
• W2410641965 cites W2120235717 @default.
• W2410641965 cites W2122273986 @default.
• W2410641965 cites W2130323443 @default.
• W2410641965 cites W2132975188 @default.
• W2410641965 cites W2135025383 @default.
• W2410641965 cites W2138006624 @default.
• W2410641965 cites W2151759421 @default.
• W2410641965 cites W2314179822 @default.
• W2410641965 cites W2325710646 @default.
• W2410641965 cites W2329049257 @default.
• W2410641965 cites W2417764438 @default.
• W2410641965 cites W2949983130 @default.
• W2410641965 doi "https://doi.org/10.1021/acschembio.6b00145" @default.
• W2410641965 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5199234" @default.
• W2410641965 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27253715" @default.
• W2410641965 hasPublicationYear "2016" @default.
• W2410641965 type Work @default.
• W2410641965 sameAs 2410641965 @default.
• W2410641965 citedByCount "19" @default.
• W2410641965 countsByYear W24106419652017 @default.
• W2410641965 countsByYear W24106419652018 @default.
• W2410641965 countsByYear W24106419652019 @default.
• W2410641965 countsByYear W24106419652020 @default.
• W2410641965 countsByYear W24106419652021 @default.
• W2410641965 countsByYear W24106419652022 @default.
• W2410641965 countsByYear W24106419652023 @default.
• W2410641965 crossrefType "journal-article" @default.
• W2410641965 hasAuthorship W2410641965A5000107010 @default.
• W2410641965 hasAuthorship W2410641965A5003287208 @default.
• W2410641965 hasAuthorship W2410641965A5022867480 @default.
• W2410641965 hasAuthorship W2410641965A5030075929 @default.
• W2410641965 hasAuthorship W2410641965A5071886170 @default.
• W2410641965 hasAuthorship W2410641965A5080450473 @default.
• W2410641965 hasAuthorship W2410641965A5087529327 @default.
• W2410641965 hasBestOaLocation W24106419652 @default.

• next