Matches in SemOpenAlex for { <https://semopenalex.org/work/W2410908424> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2410908424 endingPage "303" @default.
- W2410908424 startingPage "295" @default.
- W2410908424 abstract "Although γ-aminobutyric acid (GABA)<sub>A</sub> receptor α subunits are important for benzodiazepine (BZD) binding and GABA-current potentiation by BZDs, the presence of a γ subunit is required for high affinity BZD effects. To determine which regions unique to the γ2S subunit confer BZD binding and potentiation, we generated chimeric protein combinations of rat γ2S and α1 subunits using a modified protocol to target crossover events to the amino-terminal extracellular region of the subunits. Several chimeras with full open reading frames were constructed and placed into vectors for either voltage-clamp experiments in <i>Xenopus laevis</i>oocytes or radioligand binding experiments in human embryonic kidney 293 cells. Chimeras (χ) containing at least the amino-terminal 161 amino acids of γ2S bound BZDs with wild-type affinity when coexpressed with α1 and β2 subunits. Further analysis of the γ2S binding site region uncovered two areas, γ2S K41-W82 and γ2S R114-D161, that together are necessary and sufficient for high affinity BZD binding. Surprisingly, although the 161-amino acid residue amino terminus of the γ2S subunit is sufficient for high affinity BZD binding, it is not sufficient for efficient allosteric coupling of the GABA and BZD binding sites, as demonstrated by reduced diazepam potentiation of the GABA-gated current and GABA potentiation of [<sup>3</sup>H]flunitrazepam binding. Thus, by using γ/α chimeras, we identified two γ2 subunit regions required for BZD binding that are distinct from domain or domains responsible for allosteric coupling of the BZD and GABA binding sites." @default.
- W2410908424 created "2016-06-24" @default.
- W2410908424 creator A5062139801 @default.
- W2410908424 creator A5079209684 @default.
- W2410908424 creator A5081025343 @default.
- W2410908424 creator A5087950453 @default.
- W2410908424 date "1998-02-01" @default.
- W2410908424 modified "2023-10-03" @default.
- W2410908424 title "Molecular Dissection of Benzodiazepine Binding and Allosteric Coupling Using Chimeric γ-Aminobutyric Acid<sub>A</sub>Receptor Subunits" @default.
- W2410908424 cites W1553933303 @default.
- W2410908424 cites W1557959115 @default.
- W2410908424 cites W1565551672 @default.
- W2410908424 cites W1970739803 @default.
- W2410908424 cites W1977153009 @default.
- W2410908424 cites W1983967908 @default.
- W2410908424 cites W1988045452 @default.
- W2410908424 cites W1995188076 @default.
- W2410908424 cites W1996339077 @default.
- W2410908424 cites W1999154941 @default.
- W2410908424 cites W2003969944 @default.
- W2410908424 cites W2004050301 @default.
- W2410908424 cites W2014660497 @default.
- W2410908424 cites W2025810192 @default.
- W2410908424 cites W2048226090 @default.
- W2410908424 cites W2052725697 @default.
- W2410908424 cites W2067237230 @default.
- W2410908424 cites W2074631079 @default.
- W2410908424 cites W2092895535 @default.
- W2410908424 cites W2101625991 @default.
- W2410908424 cites W2128383909 @default.
- W2410908424 cites W2143411102 @default.
- W2410908424 cites W77926042 @default.
- W2410908424 doi "https://doi.org/10.1124/mol.53.2.295" @default.
- W2410908424 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9463488" @default.
- W2410908424 hasPublicationYear "1998" @default.
- W2410908424 type Work @default.
- W2410908424 sameAs 2410908424 @default.
- W2410908424 citedByCount "78" @default.
- W2410908424 countsByYear W24109084242012 @default.
- W2410908424 countsByYear W24109084242013 @default.
- W2410908424 countsByYear W24109084242014 @default.
- W2410908424 countsByYear W24109084242015 @default.
- W2410908424 countsByYear W24109084242016 @default.
- W2410908424 countsByYear W24109084242017 @default.
- W2410908424 countsByYear W24109084242020 @default.
- W2410908424 countsByYear W24109084242021 @default.
- W2410908424 crossrefType "journal-article" @default.
- W2410908424 hasAuthorship W2410908424A5062139801 @default.
- W2410908424 hasAuthorship W2410908424A5079209684 @default.
- W2410908424 hasAuthorship W2410908424A5081025343 @default.
- W2410908424 hasAuthorship W2410908424A5087950453 @default.
- W2410908424 hasConcept C104292427 @default.
- W2410908424 hasConcept C104317684 @default.
- W2410908424 hasConcept C107824862 @default.
- W2410908424 hasConcept C12554922 @default.
- W2410908424 hasConcept C166342909 @default.
- W2410908424 hasConcept C168258287 @default.
- W2410908424 hasConcept C170493617 @default.
- W2410908424 hasConcept C185592680 @default.
- W2410908424 hasConcept C2779414652 @default.
- W2410908424 hasConcept C515207424 @default.
- W2410908424 hasConcept C55493867 @default.
- W2410908424 hasConcept C86803240 @default.
- W2410908424 hasConceptScore W2410908424C104292427 @default.
- W2410908424 hasConceptScore W2410908424C104317684 @default.
- W2410908424 hasConceptScore W2410908424C107824862 @default.
- W2410908424 hasConceptScore W2410908424C12554922 @default.
- W2410908424 hasConceptScore W2410908424C166342909 @default.
- W2410908424 hasConceptScore W2410908424C168258287 @default.
- W2410908424 hasConceptScore W2410908424C170493617 @default.
- W2410908424 hasConceptScore W2410908424C185592680 @default.
- W2410908424 hasConceptScore W2410908424C2779414652 @default.
- W2410908424 hasConceptScore W2410908424C515207424 @default.
- W2410908424 hasConceptScore W2410908424C55493867 @default.
- W2410908424 hasConceptScore W2410908424C86803240 @default.
- W2410908424 hasIssue "2" @default.
- W2410908424 hasLocation W24109084241 @default.
- W2410908424 hasLocation W24109084242 @default.
- W2410908424 hasOpenAccess W2410908424 @default.
- W2410908424 hasPrimaryLocation W24109084241 @default.
- W2410908424 hasRelatedWork W1598309210 @default.
- W2410908424 hasRelatedWork W1976951494 @default.
- W2410908424 hasRelatedWork W1984806014 @default.
- W2410908424 hasRelatedWork W2014828185 @default.
- W2410908424 hasRelatedWork W2087153242 @default.
- W2410908424 hasRelatedWork W2095259008 @default.
- W2410908424 hasRelatedWork W2113485059 @default.
- W2410908424 hasRelatedWork W2135126224 @default.
- W2410908424 hasRelatedWork W2282615337 @default.
- W2410908424 hasRelatedWork W2987138189 @default.
- W2410908424 hasVolume "53" @default.
- W2410908424 isParatext "false" @default.
- W2410908424 isRetracted "false" @default.
- W2410908424 magId "2410908424" @default.
- W2410908424 workType "article" @default.