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• W2411330586 abstract "Research Article1 October 1985free access In vivo modulation of yeast tRNA gene expression by 5′-flanking sequences. K.C. Raymond K.C. Raymond Search for more papers by this author G.J. Raymond G.J. Raymond Search for more papers by this author J.D. Johnson J.D. Johnson Search for more papers by this author K.C. Raymond K.C. Raymond Search for more papers by this author G.J. Raymond G.J. Raymond Search for more papers by this author J.D. Johnson J.D. Johnson Search for more papers by this author Author Information K.C. Raymond, G.J. Raymond and J.D. Johnson The EMBO Journal (1985)4:2649-2656https://doi.org/10.1002/j.1460-2075.1985.tb03983.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A pentadecanucleotide sequence, TTTCAACAAATAAGT, contiguous with the 5′-end of Saccharomyces cerevisiae tRNA-Leu3 coding sequence acts as a positive modulator of transcription in a homologous in vitro system. To determine whether modulation also takes place in vivo, the amber suppressor forms of tRNA-Leu3 genes with different 5′-flanking sequences were generated by site-specific mutagenesis and cloned into YCp19, a yeast vector maintained at 1-2 copies per cell. These plasmids were transformed into S. cerevisiae strains marked with amber mutations lys2-801, met8-1, and tyr7-1. The ability of the tRNA-Leu3 amber suppressor genes (tDNA-Leu3A) to suppress functionally lys2-801 and tyr7-1 mutations in the yeast host strain correlated well with template activities measured in vitro. We conclude that the plasmid-borne tRNA gene acts as an effective suppressor from the plasmid and the conserved pentadecanucleotide sequence modulated the expression of yeast tRNA-Leu3 in vivo as well as in vitro. This regulatory sequence if found associated with genes coding for a number of tRNAs which are abundant in yeast. We postulate that this sequence represents a mechanism by which production of specific tRNAs can be enhanced to match demand created by codon use preferences. Previous ArticleNext Article Volume 4Issue 101 October 1985In this issue RelatedDetailsLoading ..." @default.
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• W2411330586 title "In vivo modulation of yeast tRNA gene expression by 5′-flanking sequences." @default.
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• W2411330586 doi "https://doi.org/10.1002/j.1460-2075.1985.tb03983.x" @default.
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