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• W2411610432 abstract "Abstract Murine high proliferative potential colony-forming cells (HPP-CFC) are known to be heterogenous with respect to proliferative capacity and in vitro responsiveness to hematopoietic growth factors. We have separated HPP-CFC into several subpopulations using counterflow centrifugal elutriation. Although HPP-CFC were identified in all of the elutriated fractions of both C3H/HeJ and C57BI/6J bone marrow cells, the distribution of HPP-CFC as well as of colony-forming units-granulocyte- macrophage (CFU-GM) in each fraction differed between these two strains of inbred mice. Six subsets of HPP-CFC were resolved that differed in growth factor responsiveness. A low-density HPP-CFC subpopulation was isolated that was distinct from day-12 spleen colony-forming units (CFU- S12), CFU-GM, and bone marrow stromal cells. This unique subpopulation of HPP-CFC is rate (3% to 9% of total HPP-CFC), appears to be lymphocyte-like in morphology, and behaves the most primitive of the HPP-CFC subsets by requiring multiple hematopoietic growth factors for optimal in vitro cloning. Further characterization of this subpopulation of HPP-CFC will determine the position of these cells in the HPP-CFC heirarchy." @default.
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• W2411610432 date "1993-07-15" @default.
• W2411610432 modified "2023-10-17" @default.
• W2411610432 title "High proliferative potential colony-forming cell heterogeneity identified using counterflow centrifugal elutriation" @default.
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• W2411610432 doi "https://doi.org/10.1182/blood.v82.2.385.385" @default.
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