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- W2411622949 abstract "Objective To investigate the effects on HIF-1α expression of rabbit adipose-derived mesenchymal stem cells (ADSCs) by dynamic compression plus IGF-1 gene transfection and explore the mechanism of promoting chondrogenesis by HIF-1α.Methods The ADSCs were harvested after the digestion of type I collagenase and transfection with pcDNA3.1-IGF-1.And then the cells were seeded onto chitosan/gelatin scaffolds with a density of 5 × 107 cells/ml and divided into groups:Group A (control),non-transfected ADSCs;Group B (IGF-1),hlGF-1 gene transfected ADSCs;Group C (loading),untransfected ADSCs with stimulation of compressive loading;Group D (loading + IGF-1),hlGF-1 gene transfected ADSCs and loading stimulation.The dynamic compression was carried out with a bio-reactor at a frequency of 0.1 Hz and a sinusoidal strain amplitude of 2% (2% at 0.1 Hz).The dynamic load was performed every 20 minutes,4 hours daily.After 7 days,morphological observation was performed.The MTT assay was used to detect the cell proliferation.Meanwhile the CM-DiI cell-labeling solution was used to observe the distribution of cells.The total amount of GAG and the expression of related genes of IGF-1,HIF-1α,type Ⅱ collagen (COL Ⅱ) and Sox-9 were quantified.Results The best morphology was found in loading + IGF-1 group.The results of proliferating capacity were:Group A ＜ C ＜ B ＜ D (P ＜ 0.01);Dil fluorescence showed that the cells were well-distributed in Group D.Meanwhile,the content of total GAG and the expression of related genes demonstrated:Group A ＜ C ＜ B ＜ D (P ＜ 0.01).And the combined effect was more significant than either alone.Conclusion In three-dimensional culture conditions,dynamic compression plus IGF-1 transfection can significantly enhance the level of autocrine IGF-1.Both of two experimental intervention factors can significantly up-regulate the mRNA level of HIF-1 α.And they exert a synergistic effect.HIF-1α plays an important role in promoting the chondrogenesis and the synthesis and secretion of extra-cellular matrix.Key words: Cartilage,articular; Stem cells; Insulin-like growth factor 1; Pressure" @default.
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- W2411622949 date "2010-12-07" @default.
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- W2411622949 title "[Experimental expression of HIF-1α in rabbit adipose-derived mesenchymal stem cells by dynamic compression plus IGF-1 gene transfection]." @default.
- W2411622949 doi "https://doi.org/10.3760/cma.j.issn.0376-2491.2010.45.015" @default.
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