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- W2411628951 abstract "Aim To construct Tat-HBV targeted ribonuclease fusion protein prokaryotic expression vector and express it in E.coli. Methods The cDNAs encoding HBV targeted ribonuclease, human eosinophil-derived neurotoxin and HBV core protein were respectively cloned into prokaryotic expression vector pTAT-HA. Recombinant plasmids were transformed into E.coli BL21(DE3) LysS, then the transformed cells were induced with IPTG. The expression of the fusion proteins were analyzed by SDS-PAGE and Western blot. Results The three recombinant plasmids were constructed and expressed after IPTG induction successfully. Conclusion The obtained Tat-HBV targeted ribonuclease fusion protein has laid the foundation for using TR in therapy of HBV infection." @default.
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- W2411628951 date "2003-01-01" @default.
- W2411628951 modified "2023-09-23" @default.
- W2411628951 title "[Construction and expression of prokaryotic expression vector for pTAT-HBV targeted ribonuclease fusion protein]." @default.
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