Matches in SemOpenAlex for { <https://semopenalex.org/work/W2411723858> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2411723858 endingPage "22" @default.
- W2411723858 startingPage "13" @default.
- W2411723858 abstract "The widespread use of human diploid fibroblasts in many tissue-culture-based systems has its origins in the pioneering work on cellular senescence by Hayflick (). He established a reliable protocol for the maintenance of fibroblast strains that was also favorable for stimulating cell proliferation. The reproducibility of the cell cultivation system he developed allowed the realization that normal diploid cells exhibit a limited proliferative potential in vitro. Depending on the age of the donor and the biopsy site, human dermal fibroblasts can reach over 50 population doublings before senescence sets in (). This high value is of great advantage to laboratories wishing to study some aspect of cell function in a normal diploid cell population. With the development of reliable cryopreservation protocols, it is possible to build up a substantial bank of early to mid passage fibroblasts from a single primary culture derived from a biopsy. This bank of stable diploid cells thus has many attributes usually only found in transformed cell lines where homogeneity of cell population over a prolonged period of time is often crucial to the experimenter. Unlike a transformed line however, human fibroblasts maintained, as described here, generally retain the normal diploid karyotype." @default.
- W2411723858 created "2016-06-24" @default.
- W2411723858 creator A5036390677 @default.
- W2411723858 creator A5057434397 @default.
- W2411723858 date "2003-11-14" @default.
- W2411723858 modified "2023-09-23" @default.
- W2411723858 title "Establishment, Maintenance, and Cloning of Human Dermal Fibroblasts" @default.
- W2411723858 cites W1996162229 @default.
- W2411723858 cites W2023913743 @default.
- W2411723858 cites W2047330128 @default.
- W2411723858 cites W2169413646 @default.
- W2411723858 doi "https://doi.org/10.1385/0-89603-441-0:13" @default.
- W2411723858 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9276257" @default.
- W2411723858 hasPublicationYear "2003" @default.
- W2411723858 type Work @default.
- W2411723858 sameAs 2411723858 @default.
- W2411723858 citedByCount "8" @default.
- W2411723858 countsByYear W24117238582015 @default.
- W2411723858 countsByYear W24117238582021 @default.
- W2411723858 countsByYear W24117238582022 @default.
- W2411723858 crossrefType "book-chapter" @default.
- W2411723858 hasAuthorship W2411723858A5036390677 @default.
- W2411723858 hasAuthorship W2411723858A5057434397 @default.
- W2411723858 hasConcept C104317684 @default.
- W2411723858 hasConcept C143191323 @default.
- W2411723858 hasConcept C1491633281 @default.
- W2411723858 hasConcept C190474072 @default.
- W2411723858 hasConcept C203014093 @default.
- W2411723858 hasConcept C2780381497 @default.
- W2411723858 hasConcept C2908647359 @default.
- W2411723858 hasConcept C522857546 @default.
- W2411723858 hasConcept C54355233 @default.
- W2411723858 hasConcept C71924100 @default.
- W2411723858 hasConcept C81885089 @default.
- W2411723858 hasConcept C86803240 @default.
- W2411723858 hasConcept C95444343 @default.
- W2411723858 hasConcept C99454951 @default.
- W2411723858 hasConceptScore W2411723858C104317684 @default.
- W2411723858 hasConceptScore W2411723858C143191323 @default.
- W2411723858 hasConceptScore W2411723858C1491633281 @default.
- W2411723858 hasConceptScore W2411723858C190474072 @default.
- W2411723858 hasConceptScore W2411723858C203014093 @default.
- W2411723858 hasConceptScore W2411723858C2780381497 @default.
- W2411723858 hasConceptScore W2411723858C2908647359 @default.
- W2411723858 hasConceptScore W2411723858C522857546 @default.
- W2411723858 hasConceptScore W2411723858C54355233 @default.
- W2411723858 hasConceptScore W2411723858C71924100 @default.
- W2411723858 hasConceptScore W2411723858C81885089 @default.
- W2411723858 hasConceptScore W2411723858C86803240 @default.
- W2411723858 hasConceptScore W2411723858C95444343 @default.
- W2411723858 hasConceptScore W2411723858C99454951 @default.
- W2411723858 hasLocation W24117238581 @default.
- W2411723858 hasLocation W24117238582 @default.
- W2411723858 hasOpenAccess W2411723858 @default.
- W2411723858 hasPrimaryLocation W24117238581 @default.
- W2411723858 hasRelatedWork W1604926853 @default.
- W2411723858 hasRelatedWork W1983614366 @default.
- W2411723858 hasRelatedWork W1987714793 @default.
- W2411723858 hasRelatedWork W2080002581 @default.
- W2411723858 hasRelatedWork W2168961821 @default.
- W2411723858 hasRelatedWork W230243712 @default.
- W2411723858 hasRelatedWork W2370286418 @default.
- W2411723858 hasRelatedWork W310985241 @default.
- W2411723858 hasRelatedWork W4236952582 @default.
- W2411723858 hasRelatedWork W2066926009 @default.
- W2411723858 isParatext "false" @default.
- W2411723858 isRetracted "false" @default.
- W2411723858 magId "2411723858" @default.
- W2411723858 workType "book-chapter" @default.