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- W2411780806 abstract "Objective: To construct a eukaryotic expression vector carrying human VEGF RNAi and to study the effect of RNA interference on VEGF expression in prostate carcinoma. Methods: VEGF RNAi was synthesized, inserted into the RNA interference eukaryotic expression vector, and confirmed by the reault sequencing. The vector was transfected into prostate cancer PC-3, the VEGF expression detected by Western blot and the cell inhibiting rate determined by MTT. Results: The VEGF RNAi eukaryotic expression vector was successfully constructed. Compared with the empty vector group and the control group, the amount of VEGF protein expression was obviously decreased in the VEGF RNAi group. The inhibiting rates were 23.5 %, 33.5 % and 40.8 % at 24, 48 and 72 h respectively. Conclusion: VEGF RNAi can inhibit the protein expression and growth of PC-3, which provides an experimental base for the biological therapy of prostate cancer." @default.
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- W2411780806 date "2006-12-01" @default.
- W2411780806 modified "2023-09-23" @default.
- W2411780806 title "[RNA interference inhibits VEGF expression and growth of PC-3 in prostate carcinoma]." @default.
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