Matches in SemOpenAlex for { <https://semopenalex.org/work/W2411806442> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2411806442 endingPage "75" @default.
- W2411806442 startingPage "66" @default.
- W2411806442 abstract "The method of anticodon loop replacement has been used to make derivatives of yeast tRNA(Phe)GmAAY with the substitution at the 37 position (tRNA(Phe)GAAA), and at both the anticodon (tRNA(Phe)GCAG) and the 37 position. A quantitative study of the interaction of various types of yeast deacylated tRNA: tRNA(Phe)GmAAY, tRNA(Phe)GAAA, tRNA(Phe)GCAG, and tRNA(Phe)-Y with the P site of the 70S ribosome.poly(U) complex was carried out at different Mg2+ concentrations and temperatures. The replacement of the Y base on the nonmodified adenosine decreases the interaction enthalpy from 39 to 24 kcal/mole, whereas the complete removal of the Y base reduces the interaction enthalpy to 16 kcal/mole. The replacement of the second letter of the anticodon (A) with cytosine leads to a drop in the enthalpy to 6 kcal/mole, which is typical of tRNA interaction with the P site in the absence of poly(U). In the absence of poly(U) the affinity of tRNA(Phe)-Y for the P site of the 70S ribosome is 5 times lower than the affinity of tRNA(Phe)GmAAY and tRNA(Phe)GCAG. Thus, in the ribosome the modified nucleotide not only stabilizes the codon-anticodon interaction owing to the stacking interaction with the stack of codon-anticodon bases, but also lowers the free energy of binding as a result of the interaction of the modified nucleotide itself with the hydrophobic center of the P site on the ribosome." @default.
- W2411806442 created "2016-06-24" @default.
- W2411806442 creator A5020988666 @default.
- W2411806442 creator A5035628947 @default.
- W2411806442 creator A5052253162 @default.
- W2411806442 creator A5074185761 @default.
- W2411806442 creator A5076760750 @default.
- W2411806442 creator A5081943882 @default.
- W2411806442 date "1994-01-01" @default.
- W2411806442 modified "2023-09-23" @default.
- W2411806442 title "[Interaction of deacylated phenylalanyl tRNA from yeasts with Escherichia coli ribosomes. The role of the modified nucleotide in codon-anticodon interaction]." @default.
- W2411806442 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8145756" @default.
- W2411806442 hasPublicationYear "1994" @default.
- W2411806442 type Work @default.
- W2411806442 sameAs 2411806442 @default.
- W2411806442 citedByCount "0" @default.
- W2411806442 crossrefType "journal-article" @default.
- W2411806442 hasAuthorship W2411806442A5020988666 @default.
- W2411806442 hasAuthorship W2411806442A5035628947 @default.
- W2411806442 hasAuthorship W2411806442A5052253162 @default.
- W2411806442 hasAuthorship W2411806442A5074185761 @default.
- W2411806442 hasAuthorship W2411806442A5076760750 @default.
- W2411806442 hasAuthorship W2411806442A5081943882 @default.
- W2411806442 hasConcept C104317684 @default.
- W2411806442 hasConcept C106774688 @default.
- W2411806442 hasConcept C153957851 @default.
- W2411806442 hasConcept C185592680 @default.
- W2411806442 hasConcept C2776023528 @default.
- W2411806442 hasConcept C2779222958 @default.
- W2411806442 hasConcept C512185932 @default.
- W2411806442 hasConcept C552990157 @default.
- W2411806442 hasConcept C55493867 @default.
- W2411806442 hasConcept C67705224 @default.
- W2411806442 hasConcept C71240020 @default.
- W2411806442 hasConcept C86803240 @default.
- W2411806442 hasConcept C88478588 @default.
- W2411806442 hasConceptScore W2411806442C104317684 @default.
- W2411806442 hasConceptScore W2411806442C106774688 @default.
- W2411806442 hasConceptScore W2411806442C153957851 @default.
- W2411806442 hasConceptScore W2411806442C185592680 @default.
- W2411806442 hasConceptScore W2411806442C2776023528 @default.
- W2411806442 hasConceptScore W2411806442C2779222958 @default.
- W2411806442 hasConceptScore W2411806442C512185932 @default.
- W2411806442 hasConceptScore W2411806442C552990157 @default.
- W2411806442 hasConceptScore W2411806442C55493867 @default.
- W2411806442 hasConceptScore W2411806442C67705224 @default.
- W2411806442 hasConceptScore W2411806442C71240020 @default.
- W2411806442 hasConceptScore W2411806442C86803240 @default.
- W2411806442 hasConceptScore W2411806442C88478588 @default.
- W2411806442 hasIssue "1" @default.
- W2411806442 hasLocation W24118064421 @default.
- W2411806442 hasOpenAccess W2411806442 @default.
- W2411806442 hasPrimaryLocation W24118064421 @default.
- W2411806442 hasRelatedWork W1964386708 @default.
- W2411806442 hasRelatedWork W1964652224 @default.
- W2411806442 hasRelatedWork W1992043883 @default.
- W2411806442 hasRelatedWork W2075359518 @default.
- W2411806442 hasRelatedWork W2142189265 @default.
- W2411806442 hasRelatedWork W2409732098 @default.
- W2411806442 hasRelatedWork W2411806442 @default.
- W2411806442 hasRelatedWork W2418320003 @default.
- W2411806442 hasRelatedWork W300760767 @default.
- W2411806442 hasRelatedWork W4290252560 @default.
- W2411806442 hasVolume "28" @default.
- W2411806442 isParatext "false" @default.
- W2411806442 isRetracted "false" @default.
- W2411806442 magId "2411806442" @default.
- W2411806442 workType "article" @default.