Matches in SemOpenAlex for { <https://semopenalex.org/work/W2411970797> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2411970797 endingPage "50" @default.
- W2411970797 startingPage "844" @default.
- W2411970797 abstract "In this study, we evaluated the efficacy of the polymerase chain reaction (PCR) in detecting urea-splitting microorganisms in desiccated urinary tract infection stones. Seventy-eight urinary tract stones were tested for the presence of Proteus mirabilis and Ureaplasma urealyticum by means of PCR with species-specific primers. Twenty-seven stone samples were composed of struvite and/or carbonate apatite (infection stone); 40 were calcium oxalate and/or calcium phosphate; seven were mixed, with struvite/carbonate apatite and calcium oxalate; and four were uric acid stones. PCR was performed with DNA extracted from pulverized stone pieces. Initial assays using the pulverized stone specimens spiked with microorganisms showed that PCR could not detect U. urealyticum at densities below 10(3) color changing units (CCU), or P. mirabilis at densities below 10(4) colony-forming units (CFU). PCR was negative for U. urealyticum and P. mirabilis in all metabolic stones from patients. P. mirabilis was detected by PCR in 10 of 34 patients with infection stones. Preoperative urine cultures grew P. mirabilis in three of these 10 patients, and were negative for P. mirabilis in the other seven. U. urealyticum was detected by PCR in stone samples from four patients, two of which were also PCR-positive for P. mirabilis. All four of these patients had infection stones: two had residual stones, and the other two had recurrence of urinary stones after their operations. These results demonstrate that microorganisms in urinary stones can be detected by PCR even when the voided urine culture is negative. Investigations into the role of bacterial infection in stone formation will require further improvements in the sensitivity of PCR assays for pathogen detection." @default.
- W2411970797 created "2016-06-24" @default.
- W2411970797 creator A5005856176 @default.
- W2411970797 creator A5046705880 @default.
- W2411970797 creator A5082018413 @default.
- W2411970797 creator A5082857859 @default.
- W2411970797 date "1999-12-01" @default.
- W2411970797 modified "2023-10-16" @default.
- W2411970797 title "Use of polymerase chain reaction to detect Proteus mirabilis and Ureaplasma urealyticum in urinary calculi." @default.
- W2411970797 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10634025" @default.
- W2411970797 hasPublicationYear "1999" @default.
- W2411970797 type Work @default.
- W2411970797 sameAs 2411970797 @default.
- W2411970797 citedByCount "3" @default.
- W2411970797 countsByYear W24119707972018 @default.
- W2411970797 countsByYear W24119707972019 @default.
- W2411970797 countsByYear W24119707972020 @default.
- W2411970797 crossrefType "journal-article" @default.
- W2411970797 hasAuthorship W2411970797A5005856176 @default.
- W2411970797 hasAuthorship W2411970797A5046705880 @default.
- W2411970797 hasAuthorship W2411970797A5082018413 @default.
- W2411970797 hasAuthorship W2411970797A5082857859 @default.
- W2411970797 hasConcept C104317684 @default.
- W2411970797 hasConcept C126322002 @default.
- W2411970797 hasConcept C2776179656 @default.
- W2411970797 hasConcept C2776672898 @default.
- W2411970797 hasConcept C2778039505 @default.
- W2411970797 hasConcept C2778838687 @default.
- W2411970797 hasConcept C2780026642 @default.
- W2411970797 hasConcept C2909217659 @default.
- W2411970797 hasConcept C49105822 @default.
- W2411970797 hasConcept C547475151 @default.
- W2411970797 hasConcept C55493867 @default.
- W2411970797 hasConcept C71924100 @default.
- W2411970797 hasConcept C77411442 @default.
- W2411970797 hasConcept C86803240 @default.
- W2411970797 hasConcept C89423630 @default.
- W2411970797 hasConceptScore W2411970797C104317684 @default.
- W2411970797 hasConceptScore W2411970797C126322002 @default.
- W2411970797 hasConceptScore W2411970797C2776179656 @default.
- W2411970797 hasConceptScore W2411970797C2776672898 @default.
- W2411970797 hasConceptScore W2411970797C2778039505 @default.
- W2411970797 hasConceptScore W2411970797C2778838687 @default.
- W2411970797 hasConceptScore W2411970797C2780026642 @default.
- W2411970797 hasConceptScore W2411970797C2909217659 @default.
- W2411970797 hasConceptScore W2411970797C49105822 @default.
- W2411970797 hasConceptScore W2411970797C547475151 @default.
- W2411970797 hasConceptScore W2411970797C55493867 @default.
- W2411970797 hasConceptScore W2411970797C71924100 @default.
- W2411970797 hasConceptScore W2411970797C77411442 @default.
- W2411970797 hasConceptScore W2411970797C86803240 @default.
- W2411970797 hasConceptScore W2411970797C89423630 @default.
- W2411970797 hasIssue "12" @default.
- W2411970797 hasLocation W24119707971 @default.
- W2411970797 hasOpenAccess W2411970797 @default.
- W2411970797 hasPrimaryLocation W24119707971 @default.
- W2411970797 hasRelatedWork W1948318411 @default.
- W2411970797 hasRelatedWork W1974241160 @default.
- W2411970797 hasRelatedWork W1998655532 @default.
- W2411970797 hasRelatedWork W2013891561 @default.
- W2411970797 hasRelatedWork W2044726291 @default.
- W2411970797 hasRelatedWork W2401019416 @default.
- W2411970797 hasRelatedWork W2403386056 @default.
- W2411970797 hasRelatedWork W2410366105 @default.
- W2411970797 hasRelatedWork W2411970797 @default.
- W2411970797 hasRelatedWork W2418852705 @default.
- W2411970797 hasVolume "98" @default.
- W2411970797 isParatext "false" @default.
- W2411970797 isRetracted "false" @default.
- W2411970797 magId "2411970797" @default.
- W2411970797 workType "article" @default.