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- W2412233842 abstract "Mesothelial cells, harvested from rat omentum, were cultured with 10% FCS/MEM till the third passage, and then seeded at a density of 2-3x10 4 /cm 2 in a 96 well micro-titer plate. Both albumin and glucose were added to each culture medium at final concentrations of 0–2 g/dl and 0–90 mM, respectively. On days 2 and 3, 5-bromo-2'-deoxy-uridine (BrdU) was added and incubated for 24-h to be incorporated into cellular DNA and detected by the ELISA principle. The lowest DNA synthesis was seen when both 2.0 g/dl of albumin and 90 mM of glucose were supplemented to the medium while the highest was obtained without albumin supplement. In the range of 0–90 mM of glucose and 0–2 g/dl of albumin, generally speaking, DNA synthesis of cultured mesothelial cells could be suppressed by either glucose or albumin, and it was greatly enhanced when both concentrations increased." @default.
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- W2412233842 date "1998-08-01" @default.
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- W2412233842 title "Suppression of DNA Synthesis of Cultured Mesothelial Cells by Supplemented Albumin" @default.
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- W2412233842 doi "https://doi.org/10.1177/039139889802100805" @default.
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