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- W2412262484 abstract "In Malaysia, the field of genomics in toxicology is still in infancy. The purpose of this study is to focus on the use of toxicogenomics for determination of gene expressions changes in cultured human fibroblast cells treated with genotoxicology free biomaterial (using Ames test), a locally produced hyroxyapatite. Dose and time response is similar to Ames test with time interval up to 21 days. mRNA is extracted, followed with RT-PCR and polyacrilamide gel electrophoresis. Changes of the gene expressions compared to the non-treated fibroblast mRNA would suggest some gene interactions in the molecule level associated with the exposure of the fibroblast cell line to the biomaterials. Further analysis (cloning & sequencing) shall be carried out to investigate the genes involved as simple changes might not signified toxicity." @default.
- W2412262484 created "2016-06-24" @default.
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- W2412262484 date "2004-05-01" @default.
- W2412262484 modified "2023-09-27" @default.
- W2412262484 title "Gene expressions screening of human cell line exposed to locally produced biomaterial." @default.
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