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- W2412634499 abstract "Transcriptomic methods make it possi-ble to take a snapshot of the processes occurring in a mixed microbial popula-tion; sampling over a time course can transform that image into a motion pic-ture. That's the goal: a molecular video of what is being done, by whom, and when, out in the environment. But there is a catch: for continuity of the frames in your movie, you need to sample the same population at each time point. When your target population is drifting effortlessly in the marine environment, and your bucket is tethered to a sluggish ship, the ocean current presents you with a new population each time you capture some water. Ed DeLong, with postdocs Liz Ottesen and Frank Ayl-ward, and colleagues from the Monterey Bay Aquarium Research Institute, solved this problem by using a free-drifting, ro-botic Environmental Sample Processor (ESP). The ESP, suspended 23 m below a drifting float on the surface in the North Pacific Subtropical Gyre (NPSG), could “go with the flow,” and collect and pre-serve samples of microbes ranging from 0.22–5 μm in size at specified time points. Physical measurements from the environment were recorded in real time, and RNA from the recovered samples was analyzed by reverse transcription and sequencing, and mapped back to the genomes of ocean denizens." @default.
- W2412634499 created "2016-06-24" @default.
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- W2412634499 date "2016-06-01" @default.
- W2412634499 modified "2023-09-25" @default.
- W2412634499 title "Small Things Considered" @default.
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- W2412634499 doi "https://doi.org/10.1128/microbe.11.286.1" @default.
- W2412634499 hasPublicationYear "2016" @default.
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