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- W2412693398 abstract "Ribosome display is a powerful method for selection of single-chain antibodies in vitro. It operates through the formation of libraries of antibody-ribosome-mRNA complexes that are selected on immobilized antigen, followed by recovery of the genetic information from the mRNA by RT-PCR. Both prokaryotic and eukaryotic versions are used. We describe our eukaryotic system, in which rabbit reticulocyte extracts are used for cell free transcription/translation and cDNA is recovered by in situ RT-PCR performed on the selected complexes." @default.
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- W2412693398 date "2008-01-01" @default.
- W2412693398 modified "2023-09-27" @default.
- W2412693398 title "Selection of Recombinant Antibodies by Eukaryotic Ribosome Display" @default.
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- W2412693398 doi "https://doi.org/10.1007/978-1-59745-398-1_13" @default.
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