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- W2412756568 abstract "ABSTRACT. Partial purification of citrus leprosis virus (CiLV) from local lesions of Cleopatramandarin was achieved using an extraction buffer of low molarity, viral precipitation with PEG-NaCl, and ultra-centrifugation in a 10 to 50% linear cesium sulfate gradient. Most of the virionsdisintegrated during the processing, but a limited number of preserved particles, about 45 to 50 × 80 to 120 nm were seen under the electron microscope. Four main bands of dsRNAs wereextracted from CiLV local lesions of Chenopodium quinoa leaves infected with the Cleopatra orsweet orange isolates, and separated by PAGE. Similar dsRNAs were also extracted from locallesions on field infected leaves of sweet orange and analyzed the same way. Values of 6 to 8 MDawere estimated. In all cases, the dsRNA bands were absent in tissue extracts from leaves notinfected with CiLV. Citrus leprosis virus (CiLV), asfar as we know, causes only locallesions but no systemic infection onsusceptible plants, and is unstablein plant sap preparations. As a con-sequence, the virus is difficult topurify (8), and that has hamperedinvestigations on its properties andon the production of antisera, thatwould be extremely useful for diag-nosis and comparison with othersimilar viruses.Evidence has been obtained thatCiLV can be mechanically transmit-ted from sweet orange and Cleopatramandarin to herbaceous hosts and,with some difficulty, from sweetorange to Caipira sweet orange (2, 7,8). Bacilliform virus particles havebeen seen in thin sections of natu-rally and experimentally infectedsweet orange plants (2, 8), and inexperimentally infected" @default.
- W2412756568 created "2016-06-24" @default.
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- W2412756568 date "2000-01-01" @default.
- W2412756568 modified "2023-09-25" @default.
- W2412756568 title "Preliminary Purification and Double Stranded RNA Analysis of Citrus Leprosis Virus" @default.
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- W2412756568 doi "https://doi.org/10.5070/c54g15f17f" @default.
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