Matches in SemOpenAlex for { <https://semopenalex.org/work/W2412786899> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2412786899 endingPage "54" @default.
- W2412786899 startingPage "47" @default.
- W2412786899 abstract "Heme and a series of synthetic heme analogs were tested for inhibition of human immunodeficiency virus-1 (HIV-1) reverse transcriptase (RT) activity. Heme and the protoporphyrin complexes of cadmium, magnesium, and tin significantly inhibited HIV-1 RT, whereas other metalloporphyrins had a lesser or no effect on the enzyme. The mechanism of inhibition was examined with respect to heme and tin protoporphyrin (SnPP), as both compounds have been utilized clinically as treatment for noninfectious disorders. Heme and SnPP inhibited HIV-1 RT in a noncompetitive manner with respect to deoxythymidine triphosphate. Inhibition depended in part on the protoporphyrin structure, because the mesoderivatives of the heme analogs essentially were without effect. Heme also markedly enhanced the inhibitory effect of azidothymidine (zidovudine, AZT) on HIV-1 RT, and the combination of the two compounds showed synergy in inhibiting HIV-1 RT. HIV-1 RT was used to reverse transcribe the glyceraldehyde phosphate dehydrogenase (GAPDH) gene from human kidney. Subsequently, GAPDH cDNA was amplified with Taq polymerase, and electrophoresis showed that HIV-1 RT catalyzed the reverse transcription of human mRNA at a rate comparable to that of Moloney murine leukemia virus. Heme and SnPP prevented cDNA synthesis by HIV-1 RT in this RT-polymerase chain reaction assay. We also examined the effects of these compounds on normal human bone marrow function. Heme stimulated both erythroid and myeloid progenitor colony formation, whereas SnPP was essentially without effect. In contrast, ZnPP had a suppressive effect on hematopoiesis. Finally, we show that heme has a sparing effect against the myelotoxicity of AZT. The results of these studies raise the possibility that combination therapy with AZT and heme, or heme plus an inhibitor of heme catabolism, might have therapeutic potential in the acquired immunodeficiency syndrome." @default.
- W2412786899 created "2016-06-24" @default.
- W2412786899 creator A5036616774 @default.
- W2412786899 creator A5041707884 @default.
- W2412786899 creator A5065853506 @default.
- W2412786899 creator A5084795749 @default.
- W2412786899 date "1996-01-01" @default.
- W2412786899 modified "2023-09-29" @default.
- W2412786899 title "Inhibition of human immunodeficiency virus-1 reverse transcriptase by heme and synthetic heme analogs." @default.
- W2412786899 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8834064" @default.
- W2412786899 hasPublicationYear "1996" @default.
- W2412786899 type Work @default.
- W2412786899 sameAs 2412786899 @default.
- W2412786899 citedByCount "10" @default.
- W2412786899 countsByYear W24127868992012 @default.
- W2412786899 countsByYear W24127868992017 @default.
- W2412786899 countsByYear W24127868992020 @default.
- W2412786899 countsByYear W24127868992022 @default.
- W2412786899 crossrefType "journal-article" @default.
- W2412786899 hasAuthorship W2412786899A5036616774 @default.
- W2412786899 hasAuthorship W2412786899A5041707884 @default.
- W2412786899 hasAuthorship W2412786899A5065853506 @default.
- W2412786899 hasAuthorship W2412786899A5084795749 @default.
- W2412786899 hasConcept C104317684 @default.
- W2412786899 hasConcept C153911025 @default.
- W2412786899 hasConcept C156719811 @default.
- W2412786899 hasConcept C159047783 @default.
- W2412786899 hasConcept C181199279 @default.
- W2412786899 hasConcept C185592680 @default.
- W2412786899 hasConcept C2522874641 @default.
- W2412786899 hasConcept C2776217839 @default.
- W2412786899 hasConcept C2778417259 @default.
- W2412786899 hasConcept C2779480358 @default.
- W2412786899 hasConcept C2780216070 @default.
- W2412786899 hasConcept C2780727368 @default.
- W2412786899 hasConcept C55493867 @default.
- W2412786899 hasConcept C67705224 @default.
- W2412786899 hasConcept C86803240 @default.
- W2412786899 hasConceptScore W2412786899C104317684 @default.
- W2412786899 hasConceptScore W2412786899C153911025 @default.
- W2412786899 hasConceptScore W2412786899C156719811 @default.
- W2412786899 hasConceptScore W2412786899C159047783 @default.
- W2412786899 hasConceptScore W2412786899C181199279 @default.
- W2412786899 hasConceptScore W2412786899C185592680 @default.
- W2412786899 hasConceptScore W2412786899C2522874641 @default.
- W2412786899 hasConceptScore W2412786899C2776217839 @default.
- W2412786899 hasConceptScore W2412786899C2778417259 @default.
- W2412786899 hasConceptScore W2412786899C2779480358 @default.
- W2412786899 hasConceptScore W2412786899C2780216070 @default.
- W2412786899 hasConceptScore W2412786899C2780727368 @default.
- W2412786899 hasConceptScore W2412786899C55493867 @default.
- W2412786899 hasConceptScore W2412786899C67705224 @default.
- W2412786899 hasConceptScore W2412786899C86803240 @default.
- W2412786899 hasIssue "1" @default.
- W2412786899 hasLocation W24127868991 @default.
- W2412786899 hasOpenAccess W2412786899 @default.
- W2412786899 hasPrimaryLocation W24127868991 @default.
- W2412786899 hasRelatedWork W1651531272 @default.
- W2412786899 hasRelatedWork W1924951592 @default.
- W2412786899 hasRelatedWork W2011954917 @default.
- W2412786899 hasRelatedWork W2022938299 @default.
- W2412786899 hasRelatedWork W2047147448 @default.
- W2412786899 hasRelatedWork W2058563055 @default.
- W2412786899 hasRelatedWork W2089024107 @default.
- W2412786899 hasRelatedWork W2094835280 @default.
- W2412786899 hasRelatedWork W2395402418 @default.
- W2412786899 hasRelatedWork W2412786899 @default.
- W2412786899 hasVolume "108" @default.
- W2412786899 isParatext "false" @default.
- W2412786899 isRetracted "false" @default.
- W2412786899 magId "2412786899" @default.
- W2412786899 workType "article" @default.