Matches in SemOpenAlex for { <https://semopenalex.org/work/W2412820327> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2412820327 endingPage "771" @default.
- W2412820327 startingPage "769" @default.
- W2412820327 abstract "AIM: To evaluate the effect of FcγRⅡB_1 (CD32) representative self-inhibitory adjustive mechanism of B cells on pathogenesis of systemic lupus erythematosus (SLE) by observing the expression characteristic and functional state of molecules on the surface of B cells from SLE patients. METHODS: The peripheral blood mononuclear cells (PBMC) were prepared by density gradient centrifugation, and the B cells were isolated from PBMC by magnetic activated cell sorting (MACS). The fluxes of intracytoplasmic calcium ([Ca~ 2+ ]_i) of B cells activated by different activators were measured by fluorescence spectrophotometric method. The IgG production by B cells cultured with activators was assayed by ELISA. The expression levels of CD32, CD19, and IgM on the surface of B cells were measured by flow cytometry. RESULTS: (1)After B cells were stimulated with goat anti-human μ chain F(ab′)_2 fragments and whole IgG respectively, the ratio of [Ca~ 2+ ]_i response by F(ab′)_2 fragments to whole IgG was significantly lower in SLE B cells compared to rheumatoid arthritis (RA) (P 0.05) or normal (P0.01) B cells. (2)The ratio of total IgG production by B cells cultured with staphylococcal protein A (SPA) to SPA plus IgG anti-μ chain was significantly lower in SLE patients compared to RA patients or normal individuals (P0.05). (3)There was no obvious difference in the expression of CD19, CD32, and IgM on the surface of B cells from SLE, RA patients and normal individuals (P0.05). CONCLUSION: The inhibitory signaling abnormality of CD32 possibly contributes to the mechanism of hyperactivity of human SLE B cells." @default.
- W2412820327 created "2016-06-24" @default.
- W2412820327 creator A5032245741 @default.
- W2412820327 creator A5047016862 @default.
- W2412820327 creator A5065596743 @default.
- W2412820327 creator A5083538338 @default.
- W2412820327 date "2006-11-01" @default.
- W2412820327 modified "2023-09-22" @default.
- W2412820327 title "[The abnormality of FcgammaRIIB1-mediated signaling and the hyperactivity of B cells from patients with systemic lupus erythematosus]." @default.
- W2412820327 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17077019" @default.
- W2412820327 hasPublicationYear "2006" @default.
- W2412820327 type Work @default.
- W2412820327 sameAs 2412820327 @default.
- W2412820327 citedByCount "0" @default.
- W2412820327 crossrefType "journal-article" @default.
- W2412820327 hasAuthorship W2412820327A5032245741 @default.
- W2412820327 hasAuthorship W2412820327A5047016862 @default.
- W2412820327 hasAuthorship W2412820327A5065596743 @default.
- W2412820327 hasAuthorship W2412820327A5083538338 @default.
- W2412820327 hasConcept C132946820 @default.
- W2412820327 hasConcept C137061746 @default.
- W2412820327 hasConcept C153911025 @default.
- W2412820327 hasConcept C159654299 @default.
- W2412820327 hasConcept C185592680 @default.
- W2412820327 hasConcept C202751555 @default.
- W2412820327 hasConcept C203014093 @default.
- W2412820327 hasConcept C2775915377 @default.
- W2412820327 hasConcept C2778453870 @default.
- W2412820327 hasConcept C2778957590 @default.
- W2412820327 hasConcept C2780942790 @default.
- W2412820327 hasConcept C553184892 @default.
- W2412820327 hasConcept C55493867 @default.
- W2412820327 hasConcept C71924100 @default.
- W2412820327 hasConcept C86803240 @default.
- W2412820327 hasConceptScore W2412820327C132946820 @default.
- W2412820327 hasConceptScore W2412820327C137061746 @default.
- W2412820327 hasConceptScore W2412820327C153911025 @default.
- W2412820327 hasConceptScore W2412820327C159654299 @default.
- W2412820327 hasConceptScore W2412820327C185592680 @default.
- W2412820327 hasConceptScore W2412820327C202751555 @default.
- W2412820327 hasConceptScore W2412820327C203014093 @default.
- W2412820327 hasConceptScore W2412820327C2775915377 @default.
- W2412820327 hasConceptScore W2412820327C2778453870 @default.
- W2412820327 hasConceptScore W2412820327C2778957590 @default.
- W2412820327 hasConceptScore W2412820327C2780942790 @default.
- W2412820327 hasConceptScore W2412820327C553184892 @default.
- W2412820327 hasConceptScore W2412820327C55493867 @default.
- W2412820327 hasConceptScore W2412820327C71924100 @default.
- W2412820327 hasConceptScore W2412820327C86803240 @default.
- W2412820327 hasIssue "6" @default.
- W2412820327 hasLocation W24128203271 @default.
- W2412820327 hasLocation W24128203272 @default.
- W2412820327 hasOpenAccess W2412820327 @default.
- W2412820327 hasPrimaryLocation W24128203271 @default.
- W2412820327 hasRelatedWork W1963848558 @default.
- W2412820327 hasRelatedWork W1984729665 @default.
- W2412820327 hasRelatedWork W2076621710 @default.
- W2412820327 hasRelatedWork W2078580589 @default.
- W2412820327 hasRelatedWork W2085126222 @default.
- W2412820327 hasRelatedWork W2088988898 @default.
- W2412820327 hasRelatedWork W2131583794 @default.
- W2412820327 hasRelatedWork W2157183976 @default.
- W2412820327 hasRelatedWork W2358399679 @default.
- W2412820327 hasRelatedWork W2364552456 @default.
- W2412820327 hasRelatedWork W2376528049 @default.
- W2412820327 hasRelatedWork W2379582001 @default.
- W2412820327 hasRelatedWork W2380403196 @default.
- W2412820327 hasRelatedWork W2399463792 @default.
- W2412820327 hasRelatedWork W2404412675 @default.
- W2412820327 hasRelatedWork W2462093997 @default.
- W2412820327 hasRelatedWork W2553638628 @default.
- W2412820327 hasRelatedWork W3030081682 @default.
- W2412820327 hasRelatedWork W3147454689 @default.
- W2412820327 hasRelatedWork W2532209026 @default.
- W2412820327 hasVolume "22" @default.
- W2412820327 isParatext "false" @default.
- W2412820327 isRetracted "false" @default.
- W2412820327 magId "2412820327" @default.
- W2412820327 workType "article" @default.