Matches in SemOpenAlex for { <https://semopenalex.org/work/W2412858277> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2412858277 endingPage "81" @default.
- W2412858277 startingPage "371" @default.
- W2412858277 abstract "In this work we have employed a new extraction buffer for isolation of cardiac lysosomes from control and ischemia canine tissue. Compared to previous techniques, this buffer (0.6 M KCl, 0.25 M sucrose) enabled a 300% increase in the yield of particulate cardiac lysosomes and allowed acceptable levels of specific activity to be maintained. It also permitted great enrichment of a membrane-bound enzyme localized to a microsomal fraction, rotenone-insensitive NADH-cytochrome c reductase (RINCR). Ischemia was produced by ligation of the left anterior descending coronary artery for 2 hr, and myocardial blood flow (MBF) was measured using 85Sr labeled microspheres (15 microns). Enzymatic changes were measured in endocardial tissue of the ischemic left ventricular wall for comparison with control nonischemic samples. N-Acetyl-beta-glucosaminidase (NAG) was the most reliable lysosomal marker enzyme; this was depleted significantly (P < 0.001) in particulate lysosomal fractions; significant increases in the supernatant (P < 0.001) were found in areas of ischemia that were less than 25% of the control MBF. Lysosomal latency also diminished significantly during ischemia. Loss of total activity of RINCR in the microsomal fraction was highly significant (P < 0.001) in the areas of profound ischemia. The above data were compared with those from animals in which methylprednisolone (30 mg/kg) was administered 30 min prior to ligation of the coronary artery. Between the two groups (untreated versus methylprednisolone-treated animals) no significant differences could be found in total losses of NAG and RINCR or the rate at which these enzymatic changes ocurred. Lysosomal latency studies also revealed lack of significant changes between both groups. Thus, no significant protective effects of methylprednisolone could be found after 2 hr of ischemia." @default.
- W2412858277 created "2016-06-24" @default.
- W2412858277 creator A5033314941 @default.
- W2412858277 creator A5069766033 @default.
- W2412858277 creator A5084022844 @default.
- W2412858277 date "1980-01-01" @default.
- W2412858277 modified "2023-09-23" @default.
- W2412858277 title "Lysosomal changes in an animal model of myocardial ischemia. Treatment with methylprednisolone." @default.
- W2412858277 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7423051" @default.
- W2412858277 hasPublicationYear "1980" @default.
- W2412858277 type Work @default.
- W2412858277 sameAs 2412858277 @default.
- W2412858277 citedByCount "0" @default.
- W2412858277 crossrefType "journal-article" @default.
- W2412858277 hasAuthorship W2412858277A5033314941 @default.
- W2412858277 hasAuthorship W2412858277A5069766033 @default.
- W2412858277 hasAuthorship W2412858277A5084022844 @default.
- W2412858277 hasConcept C126322002 @default.
- W2412858277 hasConcept C134018914 @default.
- W2412858277 hasConcept C158846371 @default.
- W2412858277 hasConcept C179437574 @default.
- W2412858277 hasConcept C181199279 @default.
- W2412858277 hasConcept C185592680 @default.
- W2412858277 hasConcept C2776820930 @default.
- W2412858277 hasConcept C2780231137 @default.
- W2412858277 hasConcept C2780372218 @default.
- W2412858277 hasConcept C541997718 @default.
- W2412858277 hasConcept C55493867 @default.
- W2412858277 hasConcept C71924100 @default.
- W2412858277 hasConcept C87644729 @default.
- W2412858277 hasConceptScore W2412858277C126322002 @default.
- W2412858277 hasConceptScore W2412858277C134018914 @default.
- W2412858277 hasConceptScore W2412858277C158846371 @default.
- W2412858277 hasConceptScore W2412858277C179437574 @default.
- W2412858277 hasConceptScore W2412858277C181199279 @default.
- W2412858277 hasConceptScore W2412858277C185592680 @default.
- W2412858277 hasConceptScore W2412858277C2776820930 @default.
- W2412858277 hasConceptScore W2412858277C2780231137 @default.
- W2412858277 hasConceptScore W2412858277C2780372218 @default.
- W2412858277 hasConceptScore W2412858277C541997718 @default.
- W2412858277 hasConceptScore W2412858277C55493867 @default.
- W2412858277 hasConceptScore W2412858277C71924100 @default.
- W2412858277 hasConceptScore W2412858277C87644729 @default.
- W2412858277 hasLocation W24128582771 @default.
- W2412858277 hasOpenAccess W2412858277 @default.
- W2412858277 hasPrimaryLocation W24128582771 @default.
- W2412858277 hasRelatedWork W1966561000 @default.
- W2412858277 hasRelatedWork W1980451061 @default.
- W2412858277 hasRelatedWork W2001509668 @default.
- W2412858277 hasRelatedWork W2015320512 @default.
- W2412858277 hasRelatedWork W2064140499 @default.
- W2412858277 hasRelatedWork W2071165980 @default.
- W2412858277 hasRelatedWork W2077297614 @default.
- W2412858277 hasRelatedWork W2094230228 @default.
- W2412858277 hasRelatedWork W2232871498 @default.
- W2412858277 hasRelatedWork W2412858277 @default.
- W2412858277 hasVolume "2" @default.
- W2412858277 isParatext "false" @default.
- W2412858277 isRetracted "false" @default.
- W2412858277 magId "2412858277" @default.
- W2412858277 workType "article" @default.