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- W2412934404 abstract "ABSTRACT Sea-urchin zygote mitotic apparatus (MA) isolated in a glycerol/dimethylsulphoxide medium were treated with pressure. Pressure treatment had no effect on spindle birefringence when MA were in full-strength isolation medium. After placing MA in quarter-strength isolation medium, pressures of 4·0 × 103– 1·8 × 104 Ibf in.−2 (2·76 × 104–1·24 × 105 kN m−2) for 15 min caused reduction of birefringence which occurred in 2 steps: firstly 20–30% of the birefringence was lost, and then, at higher pressures, the rest of the birefringence was lost. Electron microscopy suggested that pressure-induced changes were in non-microtubule material. Pressure treatment had no effect on MA isolated with hexylene glycol when the MA were pressurized in hexylene glycol; but pressure treatment did cause loss of birefringence when MA isolated in hexylene glycol were transferred immediately into glycerol/dimethylsulphoxide medium and were subsequently treated with pressure (after dilution into quarter-strength glycerol/dimethylsulphoxide). We discuss the differences in response between isolated MA and in vivo MA, and we discuss the possibility that 2 components contribute to MA birefringence." @default.
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- W2412934404 date "1976-03-01" @default.
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- W2412934404 title "Spindle Birefringence of Isolated Mitotic Apparatus Analysed by Pressure Treatment" @default.
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- W2412934404 doi "https://doi.org/10.1242/jcs.20.2.309" @default.
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