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• W2412975476 abstract "Background: The widespread multidrug-resistant Gram-negative bacilli (GNB)has necessitated the evaluation of the use of older antimicrobials such as polymyxins being the last resort drugs for the treatment of life-threatening nosocomial infections. Objectives: We conducted the present study to evaluate simple disk diffusion method (the routine method used in busy low resources hospitals)in comparison with MIC detection for polymyxin Band polymyxin E using the E-test according to the new Clinical and Laboratory Standards Institute (CLSI) guidelines and to find the level of polymyxins resistance of GNB in Kasr Alainy hospital. Methodology : A total of 50 Gram-negative bacterial isolates previously identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and classified as 12 MDR by being   resistant to three or more antimicrobial classes and38 XDR by being non-susceptible to at least one agent in all but two or fewer antimicrobial categories. Antimicrobial susceptibility testing of polymyxin B and polymyxin E (colistin) v was done by the determination of MIC by E-test, along with disk diffusion testing v. Results: Comparison of the disk diffusion and the MIC method by E- test showed100% correlation between both methods for all isolates of   Pseudomonas aeruginosa, Acinetobacter baumanii and Klebsiella spp. All isolates were sensitive to both polymyxin B and colistin with MICs and disk zone diameters within the range proposed by the CLSI 2014.MIC 50 and MIC 90 of both polymyxin B and colistin were found to be 1μg/ml. Conclusion: Polymyxins are a reliable option for the treatment of infections caused by MDR isolates of Gram-negative bacilli with proper dose adjustment. There is good concordance between the E-test and the disk diffusion. The disk diffusion method can be used for initial screening in busy hospital laboratories." @default.
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• W2412975476 date "2015-01-01" @default.
• W2412975476 modified "2023-09-27" @default.
• W2412975476 title "Polymyxins : Re-Considering the Disk Diffusion Susceptibility Testing Methodin MALDI-TOF Identified Gram Negative Bacilli" @default.
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• W2412975476 doi "https://doi.org/10.12816/0030497" @default.
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