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• W2413206710 abstract "Prolyl hydroxylase domain 2 (PHD2) enzyme, a FeII and 2-oxoglutarate (2-OG) dependent oxygenase, mediates key physiological responses to hypoxia by modulating the levels of hypoxia inducible factor 1-α (HIF1α). PHD2 has been shown to have the therapeutic potentials for conditions including anemia and ischemic disease. Currently, many activity-based assays have been developed for identifying PHD2 inhibitors. Here we report an affinity-based fluorescence polarization method using FITC-labeled HIF1α (556–574) peptide as a probe for quantitative and site-specific screening of small molecule PHD2 inhibitors." @default.
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• W2413206710 date "2015-11-12" @default.
• W2413206710 modified "2023-10-01" @default.
• W2413206710 title "Affinity-Based Fluorescence Polarization Assay for High-Throughput Screening of Prolyl Hydroxylase 2 Inhibitors" @default.
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• W2413206710 doi "https://doi.org/10.1021/acsmedchemlett.5b00394" @default.
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