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- W2413304784 abstract "We had previously found a cell-type specific DNA-binding protein of approximately 150,000 using the southwestern blot technique which was found to be a regulatory factor for activation of N-ras oncogene. In this paper, we describe a modified gel elution method for isolation of this protein using mini-slab gel electrophoresis and silver staining for identification. This method is rapid, economical and preserves the DNA-binding activity of the protein as determined by nitrocellulose filter-binding assay and mobility-shift assay. It is suitable for isolation of proteins in small quantities." @default.
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- W2413304784 date "1990-04-01" @default.
- W2413304784 modified "2023-09-23" @default.
- W2413304784 title "[Rapid isolation of cell-type specific DNA binding proteins]." @default.
- W2413304784 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2143109" @default.
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